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Western Blot (WB) (Western blot analysis using MBS604976 against HepG2 (1) and NIH/3T3 (2) cell lysate.)

Mouse anti-Human, Mouse 3-hydroxybutyrate dehydrogenase, type 1 Monoclonal Antibody | anti-BDH1 antibody

3-hydroxybutyrate dehydrogenase, type 1 (3-hydroxybutyrate dehydrogenase, BDH, BDH1, D-beta-hydroxybutyrate dehydrogenase, mitochondrial, MGC2723, MGC4347, MGC9788, SDR9C1)

Gene Names
BDH1; BDH; SDR9C1; MGC2723; MGC4347; MGC9788
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Ascites
Ascites
Synonyms
3-hydroxybutyrate dehydrogenase; type 1; Monoclonal Antibody; type 1 (3-hydroxybutyrate dehydrogenase; BDH; BDH1; D-beta-hydroxybutyrate dehydrogenase; mitochondrial; MGC2723; MGC4347; MGC9788; SDR9C1); Anti -3-hydroxybutyrate dehydrogenase; anti-BDH1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
10F603
Specificity
Recognizes human 3-hydroxybutyrate dehydrogenase, type 1 at 38kD. Species Crossreactivity: mouse.
Purity/Purification
Ascites
Ascites
Form/Format
Supplied as liquid, 0.04% sodium azide.
Applicable Applications for anti-BDH1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: ELISA: 1:10,000
Western Blot: 1:500-1:2000
Immunohistochemistry (Paraffin): 1:200-1:1000
Immunogen
Purified recombinant fragment of human 3-hydroxybutyrate dehydrogenase, type 1 expressed in E. coli.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis using MBS604976 against HepG2 (1) and NIH/3T3 (2) cell lysate.)

Western Blot (WB) (Western blot analysis using MBS604976 against HepG2 (1) and NIH/3T3 (2) cell lysate.)

Western Blot (WB)

(Western blot analysis using MBS604976 against BDH1 (aa47-343)-hIgGFc transfected HEK293 cell lysate (1).)

Western Blot (WB) (Western blot analysis using MBS604976 against BDH1 (aa47-343)-hIgGFc transfected HEK293 cell lysate (1).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human liver cancer (left) and colorectal cancer tissues (right) using H0529-18with DAB staining.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human liver cancer (left) and colorectal cancer tissues (right) using H0529-18with DAB staining.)
Related Product Information for anti-BDH1 antibody
BDH1 (3-hydroxybutyrate dehydrogenase, type 1), it is a member of the short-chain dehydrogenase/reductase gene family. This protein forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific requirement for phosphatidylcholine for optimal enzymatic activity. It catalyzes the interconversion of acetoacetate and (R)-3-hydroxybutyrate, the two major ketone bodies produced during fatty acid catabolism.
Product Categories/Family for anti-BDH1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
622
UniProt Accession #
Molecular Weight
38,157 Da
NCBI Official Full Name
3-hydroxybutyrate dehydrogenase, type 1
NCBI Official Synonym Full Names
3-hydroxybutyrate dehydrogenase, type 1
NCBI Official Symbol
BDH1
NCBI Official Synonym Symbols
BDH; SDR9C1; MGC2723; MGC4347; MGC9788
NCBI Protein Information
D-beta-hydroxybutyrate dehydrogenase, mitochondrial; OTTHUMP00000208056; OTTHUMP00000208057; OTTHUMP00000208058; OTTHUMP00000208059; (R)-3-hydroxybutyrate dehydrogenase; 3-hydroxybutyrate dehydrogenase (heart, mitochondrial); short chain dehydrogenase/reductase family 9C, member 1
UniProt Protein Name
D-beta-hydroxybutyrate dehydrogenase, mitochondrial
UniProt Gene Name
BDH1
UniProt Synonym Gene Names
BDH
UniProt Entry Name
BDH_HUMAN

NCBI Description

This gene encodes a member of the short-chain dehydrogenase/reductase gene family. The encoded protein forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific requirement for phosphatidylcholine for optimal enzymatic activity. The encoded protein catalyzes the interconversion of acetoacetate and (R)-3-hydroxybutyrate, the two major ketone bodies produced during fatty acid catabolism. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq]

Uniprot Description

BDH: a member of the short-chain dehydrogenase/reductase gene family. The encoded protein forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific requirement for phosphatidylcholine for optimal enzymatic activity. The encoded protein catalyzes the interconversion of acetoacetate and (R)-3-hydroxybutyrate, the two major ketone bodies produced during fatty acid catabolism. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]

Protein type: Mitochondrial; Lipid Metabolism - synthesis and degradation of ketone bodies; Oxidoreductase; EC 1.1.1.30; Carbohydrate Metabolism - butanoate

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: nucleoplasm; mitochondrion; mitochondrial matrix; cytoplasm; mitochondrial inner membrane

Molecular Function: 3-hydroxybutyrate dehydrogenase activity; phospholipid binding

Biological Process: response to drug; response to toxin; ketone body metabolic process; cellular lipid metabolic process; liver development; response to estradiol stimulus; response to insulin stimulus; response to starvation; response to cadmium ion; response to ethanol; ketone body catabolic process; ketone body biosynthetic process; brain development; response to nutrient; response to corticosterone stimulus

Research Articles on BDH1

Similar Products

Product Notes

The BDH1 bdh1 (Catalog #AAA604976) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The 3-hydroxybutyrate dehydrogenase, type 1 (3-hydroxybutyrate dehydrogenase, BDH, BDH1, D-beta-hydroxybutyrate dehydrogenase, mitochondrial, MGC2723, MGC4347, MGC9788, SDR9C1) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's 3-hydroxybutyrate dehydrogenase, type 1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot and Immunohistochemistry. Dilution: ELISA: 1:10,000 Western Blot: 1:500-1:2000 Immunohistochemistry (Paraffin): 1:200-1:1000. Researchers should empirically determine the suitability of the BDH1 bdh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "3-hydroxybutyrate dehydrogenase, type 1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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