Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit | MIP1b elisa kit

Monkey Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit

Cat. Num. AAA451931

• Gene Names: CCL4L1; CCL4; MIP1B; SCYA4
• Reactivity: Rhesus monkey
• Synonyms: Macrophage Inflammatory Protein 1 Beta (MIP1b); Monkey Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit; CCL4; ACT2; G-26; LAG1; MIP1-B; SCYA4; Chemokine C-C-Motif Ligand 4; Small Inducible Cytokine A4; Homologous To Mouse Mip-1b; MIP1b elisa kit

• Reactivity: Rhesus monkey
• Specificity: This assay has high sensitivity and excellent specificity for detection of MIP1b. No significant cross-reactivity or interference between MIP1b and analogues was observed.
• Sequence Length: 92

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 31.25-2000pg/mL
• Sensitivity: 9.5pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MIP1b were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MIP1b were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MIP1b elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MIP1b in monkey serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MIP1b. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MIP1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MIP1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MIP1b in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 74136311
• NCBI GeneID: 574217
• NCBI Accession #: NP_001028045.1
• NCBI GenBank Nucleotide #: NM_001032873.1
• UniProt Accession #: Q8HYQ2; Q8HYN3
• Molecular Weight: 10,103 Da
• NCBI Official Full Name: C-C motif chemokine 4
• NCBI Official Symbol: CCL4L1
• NCBI Official Synonym Symbols: CCL4; MIP1B; SCYA4
• NCBI Protein Information: C-C motif chemokine 4
• UniProt Protein Name: C-C motif chemokine 4
• Protein Family: B-box domain protein
• UniProt Gene Name: CCL4
• UniProt Synonym Gene Names: MIP1B; SCYA4; MIP-1-beta

Uniprot Description

Monokine with inflammatory and chemokinetic properties.