Monkey UBE2C ELISA Kit | UBE2C elisa kit

Monkey UBE2C (Ubiquitin Conjugating Enzyme E2C) ELISA Kit

Cat. Num. AAA2503361

• Gene Names: UBE2C; UBCH10; dJ447F3.2
• Reactivity: Monkey
• Synonyms: UBE2C; Monkey UBE2C (Ubiquitin Conjugating Enzyme E2C) ELISA Kit; UBE2C elisa kit

• Reactivity: Monkey
• Specificity: This kit recognizes natural and recombinant Monkey UBE2C. No significant cross-reactivity or interference between Monkey UBE2C and analogues was observed.
• Sequence Length: 161

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL
• Preparation and Storage: Store at 4 degree C.

Related Product Information for UBE2C elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Monkey UBE2C concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to UBE2C. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for UBE2C and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain UBE2C, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of UBE2C. You can calculate the concentration of UBE2C in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 32967283
• NCBI GeneID: 11065
• NCBI Accession #: NP_861515.1
• NCBI GenBank Nucleotide #: NM_181799.2
• UniProt Accession #: O00762; A6NP33; E1P5N7; G3XAB7
• Molecular Weight: 17,373 Da
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 C isoform 2
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2C
• NCBI Official Symbol: UBE2C
• NCBI Official Synonym Symbols: UBCH10; dJ447F3.2
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 C; cyclin-selective ubiquitin carrier protein; mitotic-specific ubiquitin-conjugating enzyme; ubiquitin-protein ligase C
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 C
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2C
• UniProt Synonym Gene Names: UBCH10
• UniProt Entry Name: UBE2C_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is required for the destruction of mitotic cyclins and for cell cycle progression, and may be involved in cancer progression. Multiple transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene have been defined on chromosomes 4, 14, 15, 18, and 19. [provided by RefSeq, Aug 2013]

Uniprot Description

UBE2C: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Belongs to the ubiquitin-conjugating enzyme family.

Protein type: Ubiquitin ligase; Ubiquitin conjugating system; Cell cycle regulation; Ligase; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: nucleoplasm; anaphase-promoting complex; cytoplasm; cytosol

Molecular Function: protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of mitotic metaphase/anaphase transition; protein ubiquitination; cyclin catabolic process; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex activation; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle; exit from mitosis; positive regulation of exit from mitosis