Monkey L-selectin ELISA Kit | L-selectin elisa kit
Monkey L-selectin ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: SELL ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SELL antibody and an SELL-HRP conjugate. The assay sample and buffer are incubated together with SELL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SELL concentration since SELL from samples and SELL-HRP conjugate compete for the anti-SELL antibody binding site. Since the number of sites is limited, as more sites are occupied by SELL from the sample, fewer sites are left to bind SELL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SELL concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a cell surface adhesion molecule that belongs to a family of adhesion/homing receptors. The encoded protein contains a C-type lectin-like domain, a calcium-binding epidermal growth factor-like domain, and two short complement-like repeats. The gene product is required for binding and subsequent rolling of leucocytes on endothelial cells, facilitating their migration into secondary lymphoid organs and inflammation sites. Single-nucleotide polymorphisms in this gene have been associated with various diseases including immunoglobulin A nephropathy. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009]
Uniprot Description
SELL: Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. Belongs to the selectin/LECAM family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 1q23-q25
Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane
Molecular Function: heparin binding; protein binding; protease binding; cell adhesion molecule binding; glycosphingolipid binding; carbohydrate binding
Biological Process: regulation of immune response; cell adhesion; blood coagulation; leukocyte migration; response to ATP
Disease: Iga Nephropathy, Susceptibility To, 1
