Monkey HHIP ELISA Kit | HHIP elisa kit

Monkey HHIP (Hedgehog Interacting Protein) ELISA Kit

Cat. Num. AAA2508652

• Gene Names: HHIP; HIP
• Reactivity: Monkey
• Synonyms: HHIP; Monkey HHIP (Hedgehog Interacting Protein) ELISA Kit; HHIP elisa kit

• Reactivity: Monkey
• Specificity: This kit recognizes natural and recombinant Monkey HHIP. No significant cross-reactivity or interference between Monkey HHIP and analogues was observed.
• Sequence Length: 700

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.313-20ng/mL
• Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL
• Preparation and Storage: Store at 4 degree C.

Related Product Information for HHIP elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Monkey HHIP concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to HHIP. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for HHIP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HHIP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of HHIP. You can calculate the concentration of HHIP in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 20143973
• NCBI GeneID: 64399
• NCBI Accession #: NP_071920.1
• NCBI GenBank Nucleotide #: NM_022475.2
• UniProt Accession #: Q96QV1; Q6PK09; Q8NCI7; Q9BXK3; Q9H1J4; Q9H7E7
• Molecular Weight: 36,373 Da
• NCBI Official Full Name: hedgehog-interacting protein
• NCBI Official Synonym Full Names: hedgehog interacting protein
• NCBI Official Symbol: HHIP
• NCBI Official Synonym Symbols: HIP
• NCBI Protein Information: hedgehog-interacting protein
• UniProt Protein Name: Hedgehog-interacting protein
• Protein Family: HHIP-like protein
• UniProt Gene Name: HHIP
• UniProt Synonym Gene Names: HIP; HHIP; HIP
• UniProt Entry Name: HHIP_HUMAN

NCBI Description

This gene encodes a member of the hedgehog-interacting protein (HHIP) family. The hedgehog (HH) proteins are evolutionarily conserved protein, which are important morphogens for a wide range of developmental processes, including anteroposterior patterns of limbs and regulation of left-right asymmetry in embryonic development. Multiple cell-surface receptors are responsible for transducing and/or regulating HH signals. The HHIP encoded by this gene is a highly conserved, vertebrate-specific inhibitor of HH signaling. It interacts with all three HH family members, SHH, IHH and DHH. Two single nucleotide polymorphisms (SNPs) near this gene are significantly associated with risk of chronic obstructive pulmonary disease (COPD). A single nucleotide polymorphism in this gene is also strongly associated with human height.[provided by RefSeq, Feb 2011]

Uniprot Description

HHIP: Modulates hedgehog signaling in several cell types including brain and lung through direct interaction with members of the hedgehog family. Belongs to the HHIP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell development/differentiation

Chromosomal Location of Human Ortholog: 4q28-q32

Cellular Component: cell surface; integral to plasma membrane; cytoplasm; extracellular region

Molecular Function: protein binding; oxidoreductase activity, acting on the CH-OH group of donors, quinone or similar compound as acceptor; zinc ion binding; quinone binding

Biological Process: smoothened signaling pathway; dorsal/ventral pattern formation; skeletal morphogenesis; negative regulation of smoothened signaling pathway; carbohydrate metabolic process; neuroblast proliferation; regulation of fibroblast growth factor receptor signaling pathway; negative regulation of signal transduction