Monkey Cell death activator CIDE-B (CIDEB) ELISA Kit | CIDEB elisa kit

Monkey Cell death activator CIDE-B (CIDEB) ELISA Kit

Cat. Num. AAA7200803

• Reactivity: Monkey
• Synonyms: Cell death activator CIDE-B (CIDEB); Monkey Cell death activator CIDE-B (CIDEB) ELISA Kit; CIDEB elisa kit

• Reactivity: Monkey
• Specificity: This assay has high sensitivity and excellent specificity for detection of CIDEB. No significant cross-reactivity or interference between CIDEB and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CIDEB and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 1.0 pg/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CIDEB elisa kit

Intended Uses: This CIDEB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey CIDEB. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: CIDEB ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CIDEB antibody and an CIDEB-HRP conjugate. The assay sample and buffer are incubated together with CIDEB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CIDEB concentration since CIDEB from samples and CIDEB-HRP conjugate compete for the anti-CIDEB antibody binding site. Since the number of sites is limited, as more sites are occupied by CIDEB from the sample, fewer sites are left to bind CIDEB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CIDEB concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 93102358
• NCBI GeneID: 27141
• NCBI Accession #: NP_055245.2
• NCBI GenBank Nucleotide #: NM_014430.2
• Molecular Weight: 24,678 Da
• NCBI Official Full Name: cell death activator CIDE-B
• NCBI Official Synonym Full Names: cell death-inducing DFFA-like effector b
• NCBI Official Symbol: CIDEB
• NCBI Protein Information: cell death activator CIDE-B
• UniProt Protein Name: Cell death activator CIDE-B
• Protein Family: Cell death activator
• UniProt Gene Name: CIDEB
• UniProt Entry Name: CIDEB_HUMAN

Uniprot Description

CIDEB: Activates apoptosis.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 14q12

Cellular Component: perinuclear region of cytoplasm; lipid particle; cytosol

Molecular Function: identical protein binding; protein binding

Biological Process: regulation of apoptosis; DNA damage response, signal transduction resulting in induction of apoptosis; apoptosis