Monkey Caprin-1 (CAPRIN1) ELISA Kit | CAPRIN1 elisa kit

Monkey Caprin-1 (CAPRIN1) ELISA Kit

Cat. Num. AAA7208634

• Gene Names: CAPRIN1; M11S1; GPIAP1; RNG105; GPIP137; GRIP137; p137GPI
• Reactivity: Monkey
• Synonyms: Caprin-1 (CAPRIN1); Monkey Caprin-1 (CAPRIN1) ELISA Kit; CAPRIN1 elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This Caprin-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey Caprin-1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CAPRIN1 elisa kit

Principle of the assay: Caprin-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Caprin-1 antibody and an Caprin-1-HRP conjugate. The assay sample and buffer are incubated together with Caprin-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Caprin-1 concentration since Caprin-1 from samples and Caprin-1-HRP conjugate compete for the anti-Caprin-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by Caprin-1 from the sample, fewer sites are left to bind Caprin-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Caprin-1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CAPRIN1 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 42734503
• NCBI GeneID: 4076
• NCBI Accession #: NP_976240.1
• NCBI GenBank Nucleotide #: NM_203364.2
• Molecular Weight: 76,862 Da
• NCBI Official Full Name: caprin-1 isoform 2
• NCBI Official Synonym Full Names: cell cycle associated protein 1
• NCBI Official Symbol: CAPRIN1
• NCBI Official Synonym Symbols: M11S1; GPIAP1; RNG105; GPIP137; GRIP137; p137GPI
• NCBI Protein Information: caprin-1; GPI-p137; caprin 1; RNA granule protein 105; GPI-anchored protein p137; GPI-anchored membrane protein 1; cell cycle-associated protein 1; activation/proliferation-associated protein 1; membrane component chromosome 11 surface marker 1; membrane component, chromosome 11, surface marker 1; cytoplasmic activation/proliferation-associated protein-1; cytoplasmic activation- and proliferation-associated protein 1
• UniProt Protein Name: Caprin-1
• Protein Family: Caprin
• UniProt Gene Name: CAPRIN1
• UniProt Synonym Gene Names: GPIAP1; GPIP137; M11S1; RNG105; GPI-p137; p137GPI
• UniProt Entry Name: CAPR1_HUMAN

Uniprot Description

CAPRIN1: May regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Belongs to the caprin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Cell surface

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: membrane; integral to plasma membrane; stress granule; dendrite; cytoplasm; cytosol

Molecular Function: RNA binding

Biological Process: negative regulation of translation; positive regulation of dendrite morphogenesis