Typical Testing Data/Standard Curve (for reference only)
Monkey Aldehyde oxidase (AOX1) ELISA Kit | AOX1 elisa kit
Monkey Aldehyde oxidase (AOX1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: AOX1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-AOX1 antibody and an AOX1-HRP conjugate. The assay sample and buffer are incubated together with AOX1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AOX1 concentration since AOX1 from samples and AOX1-HRP conjugate compete for the anti-AOX1 antibody binding site. Since the number of sites is limited, as more sites are occupied by AOX1 from the sample, fewer sites are left to bind AOX1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AOX1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Aldehyde oxidase produces hydrogen peroxide and, under certain conditions, can catalyze the formation of superoxide. Aldehyde oxidase is a candidate gene for amyotrophic lateral sclerosis. [provided by RefSeq, Jul 2008]
Uniprot Description
AOX1: Aldehyde oxidase produces hydrogen peroxide and, under certain conditions, can catalyze the formation of superoxide. Aldehyde oxidase is a candidate gene for amyotrophic lateral sclerosis. [provided by RefSeq, Jul 2008]
Protein type: EC 1.2.3.1; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Cofactor and Vitamin Metabolism - vitamin B6; Oxidoreductase; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - tryptophan
Chromosomal Location of Human Ortholog: 2q33
Cellular Component: cytoplasm; cytosol
Molecular Function: molybdopterin cofactor binding; 2 iron, 2 sulfur cluster binding; UDP-N-acetylmuramate dehydrogenase activity; electron carrier activity; FAD binding; iron ion binding; aldehyde oxidase activity; xanthine dehydrogenase activity; NAD binding
Biological Process: vitamin metabolic process; vitamin B6 metabolic process; inflammatory response; water-soluble vitamin metabolic process
Research Articles on AOX1
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Product Notes
The Monkey AOX1 aox1 (Catalog #AAA7252285) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7252285 ELISA Kit recognizes Monkey AOX1. It is sometimes possible for the material contained within the vial of "Aldehyde oxidase (AOX1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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