Monkey Agrin ELISA Kit | Agrin elisa kit

Monkey Agrin ELISA Kit

Cat. Num. AAA737332

• Gene Names: AGRN; CMSPPD
• Reactivity: Monkey
• Synonyms: Agrin; Monkey Agrin ELISA Kit; Agrin elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 0.5 -10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This AGRN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey AGRN. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Agrin elisa kit

Principle of the Assay: AGRN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AGRN antibody and an AGRN-HRP conjugate. The assay sample and buffer are incubated together with AGRN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AGRN concentration since AGRN from samples and AGRN-HRP conjugate compete for the anti-AGRN antibody binding site. Since the number of sites is limited, as more sites are occupied by AGRN from the sample, fewer sites are left to bind AGRN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGRN concentration in each sample is interpolated from this standard curve.

Product Categories/Family for Agrin elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 255738
• NCBI GeneID: 375790
• Molecular Weight: 216,733 Da
• NCBI Official Full Name: agrin, partial
• NCBI Official Synonym Full Names: agrin
• NCBI Official Symbol: AGRN
• NCBI Official Synonym Symbols: CMSPPD
• NCBI Protein Information: agrin; agrin proteoglycan
• UniProt Protein Name: Agrin
• Protein Family: Agrin
• UniProt Gene Name: AGRN
• UniProt Synonym Gene Names: AGRIN; C90; C22
• UniProt Entry Name: AGRIN_HUMAN

NCBI Description

This gene encodes one of several proteins that are critical in the development of the neuromuscular junction (NMJ), as identified in mouse knock-out studies. The encoded protein contains several laminin G, Kazal type serine protease inhibitor, and epidermal growth factor domains. Additional post-translational modifications occur to add glycosaminoglycans and disulfide bonds. In one family with congenital myasthenic syndrome affecting limb-girdle muscles, a mutation in this gene was found. [provided by RefSeq, Aug 2011]

Uniprot Description

AGRN: Plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between motor neuron and skeletal muscle. Ligand of the MUSK signaling complex that directly binds LRP4 in this complex and induces the phosphorylation of MUSK, the kinase of the complex. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Defects in AGRN are a cause of myasthenia, limb-girdle, familial (LGM). A congenital myasthenic syndrome characterized by a typical 'limb girdle' pattern of muscle weakness with small, simplified neuromuscular junctions but normal acetylcholine receptor and acetylcholinesterase function.

Protein type: Cell development/differentiation; Cell adhesion; Secreted, signal peptide; Extracellular matrix; Secreted

Chromosomal Location of Human Ortholog: 1p36.33

Cellular Component: extracellular matrix; extracellular space; lysosomal lumen; cell surface; Golgi lumen; cytoplasm; integral to membrane; extracellular region; plasma membrane; basal lamina; synapse; cell junction

Molecular Function: heparan sulfate proteoglycan binding; protein binding; structural constituent of cytoskeleton; acetylcholine receptor regulator activity; laminin binding; calcium ion binding; sialic acid binding

Biological Process: positive regulation of filopodium formation; phototransduction, visible light; axon guidance; clustering of voltage-gated sodium channels; extracellular matrix organization and biogenesis; acetylcholine receptor signaling, muscarinic pathway; glycosaminoglycan metabolic process; neurotransmitter receptor metabolic process; pathogenesis; positive regulation of synaptic growth at neuromuscular junction; signal transduction; receptor clustering; chondroitin sulfate metabolic process; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; plasma membrane organization and biogenesis; positive regulation of neuron apoptosis; carbohydrate metabolic process; synapse organization and biogenesis; positive regulation of transcription from RNA polymerase II promoter; regulation of receptor activity; retinoid metabolic process; neuromuscular junction development

Disease: Myasthenic Syndrome, Congenital, With Pre- And Postsynaptic Defects