Moloney Leukemia Virus Reverse Trancscriptase Recombinant Protein | MMLV recombinant protein
Recombinant Moloney Mouse Leukemia Virus Reverse Trancscriptase
Synonyms
Moloney Leukemia Virus Reverse Trancscriptase; Recombinant Moloney Mouse Leukemia Virus Reverse Trancscriptase; MMLV RT; Moloney Murine Leukemia Virus Reverse Trancscriptase Recombinant; MMLV recombinant protein
Host
Recombinant E Coli strain
Form/Format
50mM Tris-HCl, 0.1M NaCl, 0.1% Triton X-100, 2mM DTT, 0.1mM EDTA and 50% glycerol.
Sterile Filtered clear solution (200 U/ ul).
Sterile Filtered clear solution (200 U/ ul).
Concentration
200 units/ml. (varies by lot)
5X Reaction Buffer
250mM Tris-HCl (pH8.3), 375mM KCl, 15mM MgCl2, and 50mM DTT.
Unit Defenition
One unit is defined as the amount of enzyme required to catalyze the incorporation of 1nmol of deoxyribonucleotide into acid-insoluble forms in 10 minutes at 37 degree C, using poly(A)-oligo(dT)12-18 as the template-primer. Standard cDNA Synthesis Conditions50mM Tris-HCl (pH8.3), 75mM KCl, 3mM MgCl2, 10mM DTT, 1.0mM each dATP, dGTP, dCTP, and dTTP, 0.2 mg radom hexamer,1-5mg RNA, 200units M-MLV RT. The reaction volume was 20ml and the incubation was 45 min at 42 degree C.
Preparation and Storage
Stable for 5 days at 10 degree C, for longer period of time store at -20 degree C. Please prevent freeze-thaw cycles.
Related Product Information for MMLV recombinant protein
Description: MMLV (Moloney Murine Leukemia Virus) Reverse Transcriptase is a DNA polymerase that synthesizes a complementary DNA strands from single-stranded RNA, DNA, or an RNA-DNA hybrid as a template. This recombinant enzyme was purified from E Coli, which carried a modified MMLV-RT gene. Compared to AMV Reverse Transcriptase, this enzyme has a much weaker 5' - 3' ribonuclease H activity, which allows the syntesis of longer cDNAs (>7kb).
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Product Notes
The MMLV (Catalog #AAA142451) is a Recombinant Protein produced from Recombinant E Coli strain and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Moloney Leukemia Virus Reverse Trancscriptase, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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