MOB4A/MOB1B Recombinant Protein | MOB1B recombinant protein

MOB4A/MOB1B Protein, Human, Recombinant (GST Tag)

Cat. Num. AAA8121612

• Gene Names: MOB1B; MATS2; MOB4A; MOBKL1A
• Purity: >85% as determined by SDS-PAGE
• Synonyms: MOB4A/MOB1B; MOB4A/MOB1B Protein; Human; Recombinant (GST Tag); Human MOBKL1A/MOB1B Protein (GST Tag); MATS2 Protein; MOB4A Protein; MOBKL1A Protein; MOB kinase activator 1B; MOB1B recombinant protein

• Host: E Coli
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 150mM NaCl, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Arg216

• Species: Human
• Predicted N Terminal: Met
• Tag: N-GST
• Protein Construction: A DNA sequence encoding the human MOBKL1A (Q7L9L4)(Met1-Arg216) was fused with the GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for MOB1B recombinant protein

Background: MST1 and MST2 are the mammalian Ste2-related protein kinases most closely related to Drosophila Hippo, a major regulator of cell proliferation and survival during development. Overexpression of MST1 or MST2 in mammalian cells is proapoptotic. MST1 and MST2 activity increase during mitosis, especially in nocodazole-arrested mitotic cells, where these kinases exhibit an increase in both abundance and activation. MST1 and MST2 also can be activated nonphysiologically by okadaic acid or H2O2. The MOB1B and MOBKL1B polypeptides, homologs of the Drosophila MATS polypeptide, are identified as preferred MST1/MST2 substrates in vitro and are phosphorylated in cells in an MST1/MST2-dependent manner in mitosis and response to okadaic acid or H2O2. MST1/MST2-catalyzed MOB1B/MOBKL1B phosphorylation alters the ability of MOB1B/MOBKL1B to bind and regulate downstream targets such as the NDR-family protein kinases. Thus, MOB1B/MOBKL1B phosphorylation in cells promotes MOB1B/MOBKL1B binding to the LATS1 kinase and enables H2O2-stimulated LATS1 activation loop phosphorylation. Most importantly, the replacement of endogenous MOB1B/MOBKL1B by a non-phosphorylatable mutant is sufficient to accelerate cell proliferation substantially by speeding progression through G1/S as well as mitotic exit.

References

Ota T, et al. (2004) Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 36(1):40-5. Gerhard DS, et al. (2004) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 14(10B):2121-7. Devroe E, et al. (2004) Human Mob proteins regulate the NDR1 and NDR2 serine-threonine kinases. J Biol Chem. 279(23):24444-51. Praskova M, et al. (2008) MOB1B/MOBKL1B phosphorylation by MST1 and MST2 inhibits cell proliferation. Curr Biol. 18(5):311-21.

NCBI and Uniprot Product Information

• NCBI GI #: 348041379
• NCBI GeneID: 92597
• NCBI Accession #: NP_001231695.1
• NCBI GenBank Nucleotide #: NM_001244766.1
• UniProt Accession #: Q7L9L4; Q8IY23; B2R8U6; B4DRY3
• Molecular Weight: 25,091 Da
• NCBI Official Full Name: MOB kinase activator 1B isoform 1
• NCBI Official Synonym Full Names: MOB kinase activator 1B
• NCBI Official Symbol: MOB1B
• NCBI Official Synonym Symbols: MATS2; MOB4A; MOBKL1A
• NCBI Protein Information: MOB kinase activator 1B; mob1A; mob1 homolog 1A; MOB1 Mps One Binder homolog B; mps one binder kinase activator-like 1A; MOB1, Mps One Binder kinase activator-like 1A
• UniProt Protein Name: MOB kinase activator 1B
• Protein Family: MOB kinase activator
• UniProt Gene Name: MOB1B
• UniProt Synonym Gene Names: MOB4A; MOBKL1A; Mob1A
• UniProt Entry Name: MOB1B_HUMAN

NCBI Description

The protein encoded by this gene is similar to the yeast Mob1 protein. Yeast Mob1 binds Mps1p, a protein kinase essential for spindle pole body duplication and mitotic checkpoint regulation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

MOB1B: Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38L. Binds STK38L. Interacts with LATS1 and LATS2. Adrenal gland, bone marrow, brain, lung, placenta, prostate, salivary gland, skeletal muscle, testis, thymus, thyroid gland, uterus, colon with mucosa, fetal brain and fetal liver. Belongs to the MOB1/phocein family.

Protein type: Activator; Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: protein binding; metal ion binding; kinase activator activity; kinase binding

Biological Process: protein amino acid autophosphorylation; positive regulation of phosphorylation