MMP8 recombinant protein

MMP8, 101- 467aa, Human, His tag, E Coli (Denatured)

Cat. Num. AAA205583

• Gene Names: MMP8; HNC; CLG1; MMP-8; PMNL-CL
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: MMP8; 101- 467aa; Human; His tag; E Coli (Denatured); neutrophil collagenase preproprotein; CLG1; HNC; MMP-8; PMNL-CL; MMP8 recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 0.4M urea
• Concentration: 1mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSLTPGNPK WERTNLTYRI RNYTPQLSEA EVERAIKDAF ELWSVASPLI FTRISQGEAD INIAFYQRDH GDNSPFDGPN GILAHAFQPG QGIGGDAHFD AEETWTNTSA NYNLFLVAAH EFGHSLGLAH SSDPGALMYP NYAFRETSNY SLPQDDIDGI QAIYGLSSNP IQPTGPSTPK PCDPSLTFDA ITTLRGEILF FKDRYFWRRH PQLQRVEMNF ISLFWPSLPT GIQAAYEDFD RDLIFLFKGN QYWALSGYDI LQGYPKDISN YGFPSSVQAI DAAVFYRSKT YFFVNDQFWR YDNQRQFMEP GYPKSISGAF PGIESKVDAV FQQEHFFHVF SGPRYYAFDL IAQRVTRVAR GNKWLNCRYG
• Sequence Length: 467

• Applicable Applications for MMP8 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for MMP8 recombinant protein

MMP8 protein of the matrix metalloproteinase (MMP) family is involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is stored in secondary granules within neutrophils and is activated by autolytic cleavage. Its function is degradation of type I, II and III collagens. MMP8 is part of a cluster of MMP. Recombinant human MMP8 protein, fused to His-tag at N-terminus, was expressed in E Coli.

Product Categories/Family for MMP8 recombinant protein

Cancer

References

Hasty K.A., et al (1990). J. Biol. Chem. 265:11421-11424; Betz M., et al (1997). Eur. J. Biochem. 247:356-363;

NCBI and Uniprot Product Information

• NCBI GI #: 4505221
• NCBI GeneID: 4317
• NCBI Accession #: NP_002415
• NCBI GenBank Nucleotide #: NM_002424.2
• UniProt Accession #: P22894; Q45F99
• Molecular Weight: 44.3 kDa (390aa)
• NCBI Official Full Name: neutrophil collagenase isoform 1 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 8
• NCBI Official Symbol: MMP8
• NCBI Official Synonym Symbols: HNC; CLG1; MMP-8; PMNL-CL
• NCBI Protein Information: neutrophil collagenase
• UniProt Protein Name: Neutrophil collagenase
• Protein Family: Neutrophil collagenase
• UniProt Gene Name: MMP8
• UniProt Synonym Gene Names: CLG1; MMP-8; PMNL-CL

NCBI Description

This gene encodes a member of the matrix metalloproteinase (MMP) family of proteins. These proteins are involved in the breakdown of extracellular matrix in embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Proteolysis at different sites on this protein results in multiple active forms of the enzyme with distinct N-termini. This protein functions in the degradation of type I, II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]

Uniprot Description

Can degrade fibrillar type I, II, and III collagens.