Human Matrix metalloproteinase 2/Gelatinase A (MMP-2) ELISA Kit | MMP2 elisa kit

Human Matrix metalloproteinase 2/Gelatinase A (MMP-2) ELISA Kit

Cat. Num. AAA2802285

• Gene Names: MMP2; CLG4; MONA; CLG4A; TBE-1; MMP-II
• Reactivity: Human
• Synonyms: Matrix metalloproteinase 2/Gelatinase A (MMP-2); Human Matrix metalloproteinase 2/Gelatinase A (MMP-2) ELISA Kit; CLG4; CLG4A; MMP-II; MONA; TBE-1; collagenase type IV-A|matrix metalloproteinase 2|matrix metalloproteinase-II|neutrophil gelatinase; MMP2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human MMP2. No significant cross-reactivity or interference between Human MMP2 and analogues was observed.

• Assay Type: Sandwich
• Assay: Serum, Plasma, Other biological fluids
• Detection Range: 31.25-2000pg/mL
• Sensitivity: 12.8pg/mL
• Analysis Method: Quantitive
• Detection Wavelength: 450nm
• Sample Volume: 1-200uL
• Assay Duration: 1-4.5h
• Precision: Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Test Principle: This assay employs a two-site sandwich ELISA to quantitate MMP2 in samples. An antibody specific for MMP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMMP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Preparation and Storage: Store at 2-8 degree C. Please refer to Instruction Manual.

NCBI and Uniprot Product Information

• NCBI GI #: 189217853
• NCBI GeneID: 4313
• NCBI Accession #: NP_001121363.1
• NCBI GenBank Nucleotide #: NM_001127891.1
• UniProt Accession #: P08253; Q9UCJ8; B2R6U1; B4DWH3; E9PE45
• Molecular Weight: 73,882 Da
• NCBI Official Full Name: 72 kDa type IV collagenase isoform b
• NCBI Official Synonym Full Names: matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
• NCBI Official Symbol: MMP2
• NCBI Official Synonym Symbols: CLG4; MONA; CLG4A; TBE-1; MMP-II
• NCBI Protein Information: 72 kDa type IV collagenase; MMP-2; gelatinase A; 72 kDa gelatinase; collagenase type IV-A; neutrophil gelatinase; matrix metalloproteinase-2; matrix metalloproteinase-II
• UniProt Protein Name: 72 kDa type IV collagenase
• Protein Family: Matrix metalloproteinase
• UniProt Gene Name: MMP2
• UniProt Synonym Gene Names: CLG4A; MMP-2
• UniProt Entry Name: MMP2_HUMAN

NCBI Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

MMP2: Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Interacts (via the C-terminal hemopexin-like domains- containing region) with the integrin alpha-V/beta-3; the interaction promotes vascular invasion in angiogenic vessels and melamoma cells. Interacts (via the C-terminal PEX domain) with TIMP2 (via the C-terminal); the interaction inhibits the degradation activity. Interacts with GSK3B. Aspirin appears to inhibit expression. Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate. Inhibited by histatin-3 1/24 (histatin-5). Belongs to the peptidase M10A family.

Protein type: Cell development/differentiation; Apoptosis; Motility/polarity/chemotaxis; Protease; Secreted, signal peptide; EC 3.4.24.24; Secreted

Chromosomal Location of Human Ortholog: 16q12.2

Cellular Component: proteinaceous extracellular matrix; extracellular space; sarcomere; mitochondrion; extracellular region; plasma membrane; nucleus

Molecular Function: protein binding; zinc ion binding; metalloendopeptidase activity; serine-type endopeptidase activity

Biological Process: axon guidance; extracellular matrix organization and biogenesis; intramembranous ossification; proteolysis; blood vessel maturation; collagen catabolic process; extracellular matrix disassembly; cellular protein metabolic process; positive regulation of innate immune response; response to hypoxia; ephrin receptor signaling pathway; angiogenesis; embryo implantation

Disease: Multicentric Osteolysis, Nodulosis, And Arthropathy