Human Collagenase 3 ELISA Kit | MMP13 elisa kit

Human Collagenase 3 ELISA Kit

Cat. Num. AAA2884897

• Gene Names: MMP13; CLG3; MANDP1; MMP-13
• Reactivity: Human
• Synonyms: Collagenase 3; Human Collagenase 3 ELISA Kit; Matrix metalloproteinase-13; MMP-13; MMP13; MMP13 elisa kit

• Reactivity: Human
• Specificity: Recombinant and natural Human Collagenase 3
• Sequence Length: 471

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 0.156-10 ng/mL
• Sensitivity: <0.039 ng/mL
• Intra-assay Precision: Intra-Assay CV: <=4.8%
• Inter-assay Precision: Inter-Assay CV: <=9.6%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MMP13 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4505209
• NCBI GeneID: 4322
• NCBI Accession #: NP_002418.1
• NCBI GenBank Nucleotide #: NM_002427.3
• UniProt Accession #: P45452; Q6NWN6; A8K846; B2RCZ3
• Molecular Weight: 53,820 Da
• NCBI Official Full Name: collagenase 3 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 13
• NCBI Official Symbol: MMP13
• NCBI Official Synonym Symbols: CLG3; MANDP1; MMP-13
• NCBI Protein Information: collagenase 3
• UniProt Protein Name: Collagenase 3
• Protein Family: Collagenase
• UniProt Gene Name: MMP13
• UniProt Synonym Gene Names: MMP-13
• UniProt Entry Name: MMP13_HUMAN

NCBI Description

This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. Mutations in this gene are associated with metaphyseal anadysplasia. This gene is part of a cluster of MMP genes on chromosome 11. [provided by RefSeq, Jan 2016]

Uniprot Description

MMP13: Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process. Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO). A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age. Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1). Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia. Belongs to the peptidase M10A family.

Protein type: Secreted; Secreted, signal peptide; Protease; EC 3.4.24.-

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: extracellular region; extracellular space; Golgi apparatus; intercellular canaliculus; lysosome; proteinaceous extracellular matrix

Molecular Function: calcium ion binding; calcium-dependent protein binding; collagen binding; fibronectin binding; low-density lipoprotein receptor binding; metalloendopeptidase activity; zinc ion binding

Biological Process: bone mineralization; cellular protein metabolic process; collagen catabolic process; embryonic hindlimb morphogenesis; endochondral ossification; extracellular matrix disassembly; extracellular matrix organization and biogenesis; heart development; luteolysis; osteoblast differentiation; parturition; peptide catabolic process; proteolysis; response to drug; response to estrogen stimulus; response to hypoxia; response to mechanical stimulus

Disease: Spondyloepimetaphyseal Dysplasia, Missouri Type