MMP12 recombinant protein

Recombinant Human MMP12 Protein

Cat. Num. AAA8249455

• Gene Names: MMP12; ME; HME; MME; MMP-12
• Reactivity: Human
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Synonyms: MMP12; Recombinant Human MMP12 Protein; HME; Macrophage metalloelastase; MME; Macrophage elastase; ME; hME; Matrix metalloproteinase-12; MMP-12; MMP12 recombinant protein

• Host: Human cells
• Reactivity: Human
• Purity/Purification: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Form/Format: Supplied as a 0.2 muM filtered solution of 20mM MES, 150mM NaCl, 0.05% Brij35, pH 5.5.
• Sequence: LPLNSSTSLE KNNVLFGERY LEKFYGLEIN KLPVTKMKYS GNLMKEKIQE MQHFLGLKVT GQLDTSTLEM MHAPRCGVPD VHHFREMPGG PVWRKHYITY RINNYTPDMN REDVDYAIRK AFQVWSNVTP LKFSKINTGM ADILVVFARG AHGDFHAFDG KGGILAHAFG PGSGIGGDAH FDEDEFWTTH SGGTNLFLTA VHEIGHSLGL GHSSDPKAVM FPTYKYVDIN TFRLSADDIR GIQSLYGDPK ENQRLPNPDN SEPALCDPNL SFDAVTTVGN KIFFFKDRFF WLKVSERPKT SVNLISSLWP TLPSGIEAAY EIEARNQVFL FKDDKYWLIS NLRPEPNYPK SIHSFGFPNF VKKIDAAVFN PRFYRTYFFV DNQYWRYDER RQMMDPGYPK LITKNFQGIG PKIDAVFYSK NKYYYFFQGS NQFEYDFLLQ RITKTLKSNS WFGCVDHHHH HH
• Sequence Length: 470

• Applicable Applications for MMP12 recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for MMP12 recombinant protein

Recombinant Human MMP12 Protein is produced by our Mammalian expression system and the target gene encoding Gly106-Cys470 is expressed with a 6His tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 73858572
• NCBI GeneID: 4321
• NCBI Accession #: NP_002417.2
• NCBI GenBank Nucleotide #: NM_002426.5
• UniProt Accession #: P39900; Q2M1L9; B2R9X8; B7ZLF6
• Molecular Weight: 54,002 Da
• NCBI Official Full Name: macrophage metalloelastase preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 12
• NCBI Official Symbol: MMP12
• NCBI Official Synonym Symbols: ME; HME; MME; MMP-12
• NCBI Protein Information: macrophage metalloelastase
• UniProt Protein Name: Macrophage metalloelastase
• Protein Family: Macrophage metalloelastase
• UniProt Gene Name: MMP12
• UniProt Synonym Gene Names: HME; MME; ME; hME; MMP-12
• UniProt Entry Name: MMP12_HUMAN

NCBI Description

This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease degrades soluble and insoluble elastin. This gene may play a role in aneurysm formation and mutations in this gene are associated with lung function and chronic obstructive pulmonary disease (COPD). This gene is part of a cluster of MMP genes on chromosome 11. [provided by RefSeq, Jan 2016]

Uniprot Description

MMP12: May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3. Belongs to the peptidase M10A family.

Protein type: EC 3.4.24.65; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: extracellular region

Molecular Function: endopeptidase activity; metalloendopeptidase activity; serine-type endopeptidase activity

Biological Process: collagen catabolic process; extracellular matrix disassembly; positive regulation of epithelial cell proliferation involved in wound healing; wound healing, spreading of epidermal cells