Typical Testing Data/Standard Curve (for reference only)
Human Myeloid/lymphoid or mixed lineage leukemia 1 ELISA Kit | MLL1 elisa kit
Human Myeloid/lymphoid or mixed lineage leukemia 1 ELISA Kit
Gene Names
MLLT1; ENL; LTG19; YEATS1
Reactivity
Human
Synonyms
Myeloid/lymphoid or mixed lineage leukemia 1; Human Myeloid/lymphoid or mixed lineage leukemia 1 ELISA Kit; MLL1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of LML-1. No significant cross-reactivity or interference between LML-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LML-1 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MLL1 elisa kit
Intended Uses: This LML-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of HumanLML-1. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: LML-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-LML-1 antibody and an LML-1-HRP conjugate. The assay sample and buffer are incubated together with LML-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LML-1 concentration since LML-1 from samples and LML-1-HRP conjugate compete for the anti-LML-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by LML-1 from the sample, fewer sites are left to bind LML-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LML-1 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: LML-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-LML-1 antibody and an LML-1-HRP conjugate. The assay sample and buffer are incubated together with LML-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LML-1 concentration since LML-1 from samples and LML-1-HRP conjugate compete for the anti-LML-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by LML-1 from the sample, fewer sites are left to bind LML-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LML-1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for MLL1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
62,056 Da
NCBI Official Full Name
myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 1
NCBI Official Synonym Full Names
myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 1
NCBI Official Symbol
MLLT1
NCBI Official Synonym Symbols
ENL; LTG19; YEATS1
NCBI Protein Information
protein ENL; CTC-503J8.6; ENL/MLL fusion; MLLT1/MLL fusion; YEATS domain-containing protein 1; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog); translocated to, 1
UniProt Protein Name
Protein ENL
UniProt Gene Name
MLLT1
UniProt Synonym Gene Names
ENL; LTG19; YEATS1
UniProt Entry Name
ENL_HUMAN
Uniprot Description
MLLT1: a transcriptional regulator that can activate transcription from synthetic reporter genes in both lymphoid and myeloid cells. A chromosomal aberration involving MLLT1 is associated with acute leukemias.
Protein type: Oncoprotein; Transcription regulation
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: nucleoplasm; transcription elongation factor complex; cytoplasm; nucleolus; nucleus
Molecular Function: protein binding; DNA binding
Biological Process: transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; negative regulation of protein kinase activity
Research Articles on MLL1
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Product Notes
The Human MLL1 mllt1 (Catalog #AAA754904) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA754904 ELISA Kit recognizes Human MLL1. It is sometimes possible for the material contained within the vial of "Myeloid/lymphoid or mixed lineage leukemia 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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