Western Blot (WB)
(Western blot analysis of extract from K562 cells, using Myosin Light Chain 2 (Ab-18) antibody.)
Myosin Light Chain 2 Antibody | anti-MLC-2 antibody
Myosin Light Chain 2 (Ab-18) Antibody
Gene Names
MYL12A; MLCB; MRCL3; MRLC3; MYL2B; MLC-2B; HEL-S-24
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
Myosin Light Chain 2; Myosin Light Chain 2 (Ab-18) Antibody; anti-MLC-2 antibody
Reactivity
Human, Mouse, Rat
Specificity
Myosin Light Chain 2 (Ab-18) antibody detects endogenous levels of total Myosin Light Chain 2 protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
171
Applicable Applications for anti-MLC-2 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from mouse Myosin Light Chain 2 around the phosphorylation site of serine 18 (A-T-SP-N-V).
Preparation and Storage
Store at-20 degree C for 1 year
Western Blot (WB)
(Western blot analysis of extract from K562 cells, using Myosin Light Chain 2 (Ab-18) antibody.)
Western Blot (WB)
(Western blot analysis of extract from K562 cells, using Myosin Light Chain 2 (Ab-18) antibody.)
Product Categories/Family for anti-MLC-2 antibody
References
Janiak A, et al. (2006) Mol Biol Cell. Apr; 17(4): 1606-1619. Croft DR, et al. (2006) Mol Cell Biol. 2 Jun; 26(12): 4612-4627 Li Z, et al. (2006) Mol Cell Biol. Jun; 26(11): 4240-4256.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,794 Da
NCBI Official Full Name
myosin regulatory light chain 12A isoform 1
NCBI Official Synonym Full Names
myosin light chain 12A
NCBI Official Symbol
MYL12A
NCBI Official Synonym Symbols
MLCB; MRCL3; MRLC3; MYL2B; MLC-2B; HEL-S-24
NCBI Protein Information
myosin regulatory light chain 12A
UniProt Protein Name
Myosin regulatory light chain 12A
Protein Family
UniProt Gene Name
MYL12A
UniProt Synonym Gene Names
MLCB; MRLC3; RLC; HEL-S-24
NCBI Description
This gene encodes a nonsarcomeric myosin regulatory light chain. This protein is activated by phosphorylation and regulates smooth muscle and non-muscle cell contraction. This protein may also be involved in DNA damage repair by sequestering the transcriptional regulator apoptosis-antagonizing transcription factor (AATF)/Che-1 which functions as a repressor of p53-driven apoptosis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 8.[provided by RefSeq, Dec 2014]
Uniprot Description
Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion ().
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Product Notes
The MLC-2 myl12a (Catalog #AAA857564) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Myosin Light Chain 2 (Ab-18) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Myosin Light Chain 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the MLC-2 myl12a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Myosin Light Chain 2, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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