Probable inorganic polyphosphate/ATP-NAD kinase (ppnK), partial Recombinant Protein | ppnK recombinant protein

Recombinant Methanocaldococcus jannaschii Probable inorganic polyphosphate/ATP-NAD kinase (ppnK), partial

• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Probable inorganic polyphosphate/ATP-NAD kinase (ppnK); partial; Recombinant Methanocaldococcus jannaschii Probable inorganic polyphosphate/ATP-NAD kinase (ppnK); Probable inorganic polyphosphate/ATP-NAD kinase; Poly(P)/ATP NAD kinase; EC=2.7.1.23; ppnK recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)

• Species: Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 15669107
• NCBI GeneID: 1451806
• NCBI Accession #: NP_247912.1
• NCBI GenBank Nucleotide #: NC_000909.1
• UniProt Accession #: Q58327
• Molecular Weight: 64,119 Da
• NCBI Official Full Name: inorganic polyphosphate/ATP-NAD kinase
• NCBI Official Symbol: pnk
• NCBI Protein Information: inorganic polyphosphate/ATP-NAD kinase
• UniProt Protein Name: Bifunctional NADP phosphatase/NAD kinase
• UniProt Gene Name: MJ0917
• UniProt Synonym Gene Names: NADPase; pNPPase
• UniProt Entry Name: NPPNK_METJA

Uniprot Description

Involved in the regulation of the intracellular balance between NAD(H) and NADP(H), and is a key enzyme in the biosynthesis of NADP. Catalyzes the phosphorylation and dephosphorylation of NAD and NADP, respectively. Although it shows conflicting dual activities and is able to supply NADP, it seems that its physiological role is to prevent excess accumulation of NADP. Kinase can use ATP and other nucleoside triphosphates (UTP, TTP, CTP, GTP) as well as inorganic polyphosphate (poly(P)) as phosphoryl donors, however poly(P) is not considered to be the physiological phosphoryl donor. NAD is the preferred substrate for the kinase, but NADH can also be used as phosphoryl acceptor. Phosphatase can use NADP or NADPH as phosphoryl donor, but NADP is the preferred substrate. Phosphatase also has an activity toward the terminal phosphate group at C-2 of adenosine in 2'-AMP and toward the phosphate group at C-1 of fructose 1,6-bisphosphate, but not toward inositol 1-phosphate.