SDS-Page
MGAT2 recombinant protein
MGAT2, 30-447aa, Human, His-tag, Baculovirus
Gene Names
MGAT2; GNT2; CDG2A; CDGS2; GNT-II; GLCNACTII
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
MGAT2; 30-447aa; Human; His-tag; Baculovirus; Alpha-1; 6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase; CDG2A; CDGS2; GLCNACTII; GNT-II; GNT2; MGAT2 recombinant protein
Host
Baculovirus
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl (pH 7.5) containing 10% glycerol, 0.15M NaCl, 1mM DTT.
Concentration
0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence
ADPRQRKNEA LAPPLLDAEP ARGAGGRGGD HPSVAVGIRR VSNVSAASLV PAVPQPEADN LTLRYRSLVY QLNFDQTLRN VDKAGTWAPR ELVLVVQVHN RPEYLRLLLD SLRKAQGIDN VLVIFSHDFW STEINQLIAG VNFCPVLQVF FPFSIQLYPN EFPGSDPRDC PRDLPKNAAL KLGCINAEYP DSFGHYREAK FSQTKHHWWW KLHFVWERVK ILRDYAGLIL FLEEDHYLAP DFYHVFKKMW KLKQQECPEC DVLSLGTYSA SRSFYGMADK VDVKTWKSTE HNMGLALTRN AYQKLIECTD TFCTYDDYNW DWTLQYLTVS CLPKFWKVLV PQIPRIFHAG DCGMHHKKTC RPSTQSAQIE SLLNNNKQYM FPETLTISEK FTVVAISPPR KNGGWGDIRD HELCKSYRRL QHHHHHH
Sequence Length
447
Applicable Applications for MGAT2 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Endotoxin Level
< 1.0 EU per 1ug of protein (determined by LAL method)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for MGAT2 recombinant protein
MGAT2, also known as alpha-1, 6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, is a single-pass type 2 membrane protein. It has the typical glycosyltransferase domains that composes a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain, and a C-terminal catalytic domain. This protein catalyzes an essential step in the conversion of oligo-mannose to complex N-glycans. Sometimes mutations in this gene may lead to carbohydrate-deficient glycoprotein syndrome, type II. Recombinant human MGAT2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Categories/Family for MGAT2 recombinant protein
References
Tan J., et al, (1996) Am. J. Hum. Genet. 59:810-817. Maszczak-Seneczko D., et al, (2015) J. Biol. Chem. 290:15475-15486.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
49.3kDa (427aa) 40-57kDa (SDS-PAGE under reducing conditions.)
NCBI Official Full Name
alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
NCBI Official Synonym Full Names
mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
NCBI Official Symbol
MGAT2
NCBI Official Synonym Symbols
GNT2; CDG2A; CDGS2; GNT-II; GLCNACTII
NCBI Protein Information
alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
UniProt Protein Name
Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
UniProt Gene Name
MGAT2
UniProt Synonym Gene Names
GNT-II
NCBI Description
The product of this gene is a Golgi enzyme catalyzing an essential step in the conversion of oligomannose to complex N-glycans. The enzyme has the typical glycosyltransferase domains: a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain, and a C-terminal catalytic domain. Mutations in this gene may lead to carbohydrate-deficient glycoprotein syndrome, type II. The coding region of this gene is intronless. Transcript variants with a spliced 5' UTR may exist, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
Uniprot Description
Catalyzes an essential step in the conversion of oligo-mannose to complex N-glycans.
Research Articles on MGAT2
Similar Products
Product Notes
The MGAT2 mgat2 (Catalog #AAA206819) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's MGAT2 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the MGAT2 mgat2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ADPRQRKNEA LAPPLLDAEP ARGAGGRGGD HPSVAVGIRR VSNVSAASLV PAVPQPEADN LTLRYRSLVY QLNFDQTLRN VDKAGTWAPR ELVLVVQVHN RPEYLRLLLD SLRKAQGIDN VLVIFSHDFW STEINQLIAG VNFCPVLQVF FPFSIQLYPN EFPGSDPRDC PRDLPKNAAL KLGCINAEYP DSFGHYREAK FSQTKHHWWW KLHFVWERVK ILRDYAGLIL FLEEDHYLAP DFYHVFKKMW KLKQQECPEC DVLSLGTYSA SRSFYGMADK VDVKTWKSTE HNMGLALTRN AYQKLIECTD TFCTYDDYNW DWTLQYLTVS CLPKFWKVLV PQIPRIFHAG DCGMHHKKTC RPSTQSAQIE SLLNNNKQYM FPETLTISEK FTVVAISPPR KNGGWGDIRD HELCKSYRRL QHHHHHH. It is sometimes possible for the material contained within the vial of "MGAT2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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