MERTK / Mer recombinant protein

Recombinant Human MERTK / Mer Protein GST tag

Cat. Num. AAA2545117

• Gene Names: MERTK; MER; RP38; c-Eyk; c-mer; Tyro12
• Purity: > 92 % as determined by SDS-PAGE
• Synonyms: MERTK / Mer; Recombinant Human MERTK / Mer Protein GST tag; MER; MGC133349; RP38; c-mer; MERTK; MERTK / Mer recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 92 % as determined by SDS-PAGE
• Form/Format: Supplied as sterile 50mM Tris, 100mM NaCl, pH 7.4, 20% gly, 0.3mM DTT
• Sequence Length: 999

• Application Notes: The recombinant human MERTK (aa578-872)/GST chimera consists of 532 amino acids and has a calculated molecular mass of 62 kDa. It migrates as an approximately 50 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for MERTK / Mer recombinant protein

Background: Proto-oncogene tyrosine-protein kinase MER (MERTK) is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type -III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. MERTK is localized in membrane and is no expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines. This protein is highly expressed in testis, ovary, prostate, lung, and kidney, with lower expression in spleen, small intestine, colon, and liver. MERTK regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. MERTK plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3. Defects in MERTK are the cause of retinitis pigmentosa type 38.

Description: A DNA sequence encoding the human MERTK (Q12866) protein kinase domain (Glu 578-Tyr 872) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 66932918
• NCBI GeneID: 10461
• NCBI Accession #: NP_006334.2
• NCBI GenBank Nucleotide #: NM_006343.2
• UniProt Accession #: Q12866; Q9HBB4
• Molecular Weight: 110,249 Da
• NCBI Official Full Name: tyrosine-protein kinase Mer
• NCBI Official Synonym Full Names: MER proto-oncogene, tyrosine kinase
• NCBI Official Symbol: MERTK
• NCBI Official Synonym Symbols: MER; RP38; c-Eyk; c-mer; Tyro12
• NCBI Protein Information: tyrosine-protein kinase Mer
• UniProt Protein Name: Tyrosine-protein kinase Mer
• UniProt Gene Name: MERTK
• UniProt Synonym Gene Names: MER

NCBI Description

This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. Mutations in this gene have been associated with disruption of the retinal pigment epithelium (RPE) phagocytosis pathway and onset of autosomal recessive retinitis pigmentosa (RP). [provided by RefSeq, Jul 2008]

Uniprot Description

Mer: a proto-oncogene receptor tyrosine-protein kinase of the Axl family. Binds several ligands including LGALS3, Tubby, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3

Protein type: Axl family; EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Oncoprotein; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Chromosomal Location of Human Ortholog: 2q13

Cellular Component: cytoplasm; extracellular space; integral to plasma membrane; plasma membrane

Molecular Function: protein binding

Biological Process: cell surface receptor linked signal transduction; cell-cell signaling; leukocyte migration; phagocytosis; positive regulation of phagocytosis; protein amino acid phosphorylation

Disease: Retinitis Pigmentosa 38