Myocyte Specific Enhancer Factor 2A Recombinant Protein | MEF2A recombinant protein

Recombinant Myocyte Specific Enhancer Factor 2A (MEF2A)

Cat. Num. AAA2033516

• Gene Names: MEF2A; mef2; RSRFC4; RSRFC9
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Myocyte Specific Enhancer Factor 2A; Recombinant Myocyte Specific Enhancer Factor 2A (MEF2A); MEF2-A; ADCAD1; RSRFC4; RSRFC9; MADS Box Transcription Enhancer Factor 2; Polypeptide A; Serum response factor-like protein 1; MEF2A recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: MGRKKIQITR IMDERNRQVT FTKRKFGLMK KAYELSVLCD CEIALIIFNS SNKLFQYAST DMDKVLLKYT EYNEPHESRT NSDIVETLRK KGLNGCESPD ADDYFEHSPL SEDRFSKLNEDSDFIFKRGP PGLPPQNFSM SVTVPVTSPN ALSYTNPGSS
• Sequence Length: 499

• Applicable Applications for MEF2A recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 22.0kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Met1~Ser160
• Organism Species: Mouse
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 195972799
• NCBI GeneID: 4205
• NCBI Accession #: NP_005578.2
• NCBI GenBank Nucleotide #: NM_005587.3
• Molecular Weight: 45,570 Da
• NCBI Official Full Name: myocyte-specific enhancer factor 2A isoform 1
• NCBI Official Synonym Full Names: myocyte enhancer factor 2A
• NCBI Official Symbol: MEF2A
• NCBI Official Synonym Symbols: mef2; RSRFC4; RSRFC9
• NCBI Protein Information: myocyte-specific enhancer factor 2A
• UniProt Protein Name: Myocyte-specific enhancer factor 2A
• Protein Family: Myocyte-specific enhancer factor
• UniProt Gene Name: MEF2A
• UniProt Synonym Gene Names: MEF2
• UniProt Entry Name: MEF2A_HUMAN

NCBI Description

The protein encoded by this gene is a DNA-binding transcription factor that activates many muscle-specific, growth factor-induced, and stress-induced genes. The encoded protein can act as a homodimer or as a heterodimer and is involved in several cellular processes, including muscle development, neuronal differentiation, cell growth control, and apoptosis. Defects in this gene could be a cause of autosomal dominant coronary artery disease 1 with myocardial infarction (ADCAD1). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2010]

Uniprot Description

MEF2A: a myocyte-specific enhancing transcription factor which binds specifically to the MEF2 element present in the regulatory regions of many, if not all, muscle-specific genes. A member of the MADS gene family that also includes several homeotic genes and other transcription factors, all of which share a conserved DNA-binding domain. Five alternatively spliced isoforms have been reported.

Protein type: Motility/polarity/chemotaxis; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 15q26

Cellular Component: cytoplasm; nuclear chromatin; nucleoplasm; nucleus; transcription factor complex

Molecular Function: chromatin binding; histone acetyltransferase binding; histone deacetylase binding; protein binding; protein heterodimerization activity; protein kinase binding; RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; SMAD binding; transcription activator binding; transcription factor activity

Biological Process: apoptosis; dendrite morphogenesis; heart development; innate immune response; MAPKKK cascade; mitochondrial genome maintenance; mitochondrion distribution; muscle cell differentiation; muscle development; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; nerve growth factor receptor signaling pathway; positive regulation of muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; stress-activated MAPK cascade; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway; transcription from RNA polymerase II promoter; transcription, DNA-dependent; ventricular cardiac myofibril development

Disease: Coronary Artery Disease, Autosomal Dominant, 1