MED4 recombinant protein

MED4, 1-270aa, uman, His tag, E Coli

Cat. Num. AAA203489

• Gene Names: MED4; ARC36; VDRIP; DRIP36; TRAP36; HSPC126
• Applications: SDS-Page
• Purity: > 80% by SDS-PAGE
• Synonyms: MED4; 1-270aa; uman; His tag; E Coli; Mediator complex subunit 4; ARC36; DRIP36; VDRIP; Mediator complex subunit 4 Activator-recruited cofactor 36 kDa component; HSPC126; Mediator of RNA polymerase II transcription subunit 4; TRAP/SMCC/PC2 subunit p36 subunit; Vitamin D3 receptor-interacting protein complex 36 kDa component.; MED4 recombinant protein

• Host: E Coli
• Purity/Purification: > 80% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 1mM DTT, 100mM NaCl
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MAASSSGEKE KERLGGGLGV AGGNSTRERL LSALEDLEVL SRELIEMLAI SRNQKLLQAG EENQVLELLI HRDGEFQELM KLALNQGKIH HEMQVLEKEV EKRDGDIQQL QKQLKEAEQI LATAVYQAKE KLKSIEKARK GAISSEEIIK YAHRISASNA VCAPLTWVPG DPRRPYPTDL EMRSGLLGQM NNPSTNGVNG HLPGDALAAG RLPDVLAPQY PWQSNDMSMN MLPPNHSSDF LLEPPGHNKE DEDDVEIMST DSSSSSSESD LEHHHHHH
• Sequence Length: 270

• Applicable Applications for MED4 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for MED4 recombinant protein

Mediator complex subunit 4 (MED4) is also known as mediator of RNA polymerase II transcription subunit 4 or vitamin D3 receptor-interacting protein complex 36 kDa component (DRIP36). This protein is a component of the vitamin D receptor-interacting protein (DRIP) complex which functions as a nuclear receptor coactivator. The DRIP complex is capable of activating nuclear receptors in a ligand-dependent manner. Recombinant human MED4, fused to His-tag at C-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for MED4 recombinant protein

Transcription & Translation

References

Rachez C., et al. (1999) Nature 398 (6730): 824-8.; Panierakis C., et al. (2009) Int J Infect Dis.13 (6):e437-43.;

NCBI and Uniprot Product Information

• NCBI GI #: 13528774
• NCBI GeneID: 29079
• Molecular Weight: 30.7kDa (278aa), confirmed by MALDI-TOF
• NCBI Official Full Name: Mediator complex subunit 4
• NCBI Official Synonym Full Names: mediator complex subunit 4
• NCBI Official Symbol: MED4
• NCBI Official Synonym Symbols: ARC36; VDRIP; DRIP36; TRAP36; HSPC126
• NCBI Protein Information: mediator of RNA polymerase II transcription subunit 4
• UniProt Protein Name: Mediator of RNA polymerase II transcription subunit 4
• Protein Family: Mediator of RNA polymerase II transcription
• UniProt Gene Name: MED4
• UniProt Synonym Gene Names: ARC36; DRIP36; VDRIP; ARC36; DRIP36

NCBI Description

This gene encodes a component of the Mediator complex. The Mediator complex interacts with DNA-binding gene-specific transcription factors to modulate transcription by RNA polymerase II. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.