Histone-lysine N-methyltransferase MEDEA (MEA) Recombinant Protein | MEA recombinant protein

Recombinant Arabidopsis thaliana Histone-lysine N-methyltransferase MEDEA (MEA) , partial

• Gene Names: MEA; EMB173; EMBRYO DEFECTIVE 173; FERTILIZATION INDEPENDENT SEED 1; FIS1; MEDEA; SDG5; SET DOMAIN-CONTAINING PROTEIN 5; T14P4.11; T14P4_11
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Histone-lysine N-methyltransferase MEDEA (MEA); Recombinant Arabidopsis thaliana Histone-lysine N-methyltransferase MEDEA (MEA); partial; MEA recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Length: 689

• Species: Arabidopsis thaliana (Mouse-ear cress)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 18378985
• NCBI GeneID: 839422
• NCBI Accession #: NP_563658.1
• NCBI GenBank Nucleotide #: NM_100139.4
• UniProt Accession #: O65312
• Molecular Weight: 79,310 Da
• NCBI Official Full Name: SET domain-containing protein
• NCBI Official Symbol: MEA
• NCBI Official Synonym Symbols: EMB173; EMBRYO DEFECTIVE 173; FERTILIZATION INDEPENDENT SEED 1; FIS1; MEDEA; SDG5; SET DOMAIN-CONTAINING PROTEIN 5; T14P4.11; T14P4_11
• NCBI Protein Information: SET domain-containing protein
• UniProt Protein Name: Histone-lysine N-methyltransferase MEDEA
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: MEA
• UniProt Synonym Gene Names: EMB173; FIS1; MEDEA; SDG5; SET5

NCBI Description

Encodes a putative transcription factor MEDEA (MEA) that negatively regulates seed development in the absence of fertilization. Mutations in this locus result in embryo lethality. MEA is a Polycomb group gene that is imprinted in the endosperm. The maternal allele is expressed and the paternal allele is silent. MEA is controlled by DEMETER (DME), a DNA glycosylase required to activate MEA expression, and METHYLTRANSFERASE I (MET1), which maintains CG methylation at the MEA locus. MEA is involved in the negative regulation of its own imprinted gene expression; the effect is not only allele-specific but also dynamically regulated during seed development. In the ovule, the MEA transcripts are accumulated at their highest level before fertilization and gradually decrease after fertilization

Uniprot Description

Polycomb group (PcG) protein. Catalytic subunit of some PcG multiprotein complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target genes. Required to prevent the proliferation of the central cell of the female gametophyte by repressing target genes before fertilization. After fertilization, it probably also regulates the embryo and endosperm proliferation and anteroposterior organization during seed development. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. Interacts with the promoter and repress the transcription of genes such as PHE1 and PHE2, that are paternally active and maternally silenced genes.