Midkine (MK) Recombinant Protein | MK recombinant protein

Recombinant Midkine (MK)

Cat. Num. AAA2010039

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Midkine (MK); Recombinant Midkine (MK); MK recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.; MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- AKKKDKVKK GSECSEWTWG PCTPSSKDCG MGFREGTCGA QTQRIHCKVP CNWKKEFGAD CKYKFESWGA CDGSTGTKAR QGTLKKARYN AQCQETIRVT KPCTSKTKSK AKAKKGKGKD
• Sequence Length: 140

• Applicable Applications for MK recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Ala22~Asp140 (Accession # Q9R1S9) with two N-terminal Tags, His-tag and S-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 13540681
• NCBI GeneID: 81517
• NCBI Accession #: NP_110486.1
• NCBI GenBank Nucleotide #: NM_030859.2
• UniProt Accession #: Q9R1S9
• Molecular Weight: 18.8kDa
• NCBI Official Full Name: midkine
• NCBI Official Synonym Full Names: midkine
• NCBI Official Symbol: Mdk
• NCBI Protein Information: midkine; MK
• UniProt Protein Name: Midkine
• Protein Family: Midkine
• UniProt Gene Name: Mdk
• UniProt Synonym Gene Names: MK
• UniProt Entry Name: MK_RAT

NCBI Description

heparin-binding growth factor; involved in early fetal development of the rat adrenal [RGD, Feb 2006]

Uniprot Description

Function: Developmentally regulated, secreted growth factor homologous to pleiotrophin (PTN), which has heparin binding activity. Binds anaplastic lymphoma kinase (ALK) which induces ALK activation and subsequent phosphorylation of the insulin receptor substrate (IRS1), followed by the activation of mitogen-activated protein kinase (MAPK) and PI3-kinase, and the induction of cell proliferation. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development

By similarity. Ref.1 Ref.2

Subunit structure: Homodimer

By similarity. Interacts with ALK

By similarity.

Subcellular location: Secreted

Probable.

Tissue specificity: Expressed at a low level in arteries, and at higher levels in newly formed neointima. In brain, expressed in the caudate nucleus and the brain stem. Ref.1 Ref.3

Developmental stage: In the cortices of the adrenal glands, expressed at 12.5 dpc, decreases considerably at 15.5 dpc and is almost undetectable in the newborn stage. In brain, expressed at low levels in early embryos, expression peaks between E12 and E14, and rapidly falls until birth when expression is barely detectable. Ref.2 Ref.3

Induction: In arteries 3 days after injury. Expression continues to increase until day 7 after injury and decreases slightly by day 14. Ref.1

Sequence similarities: Belongs to the pleiotrophin family.