Human Myelin ELISA Kit | MBP elisa kit

Human Myelin basic protein (MBP) ELISA Kit

Cat. Num. AAA285794

• Reactivity: Human
• Synonyms: Myelin; Human Myelin basic protein (MBP) ELISA Kit; MGC99675; Golli-mbp|OTTHUMP00000174383; MBP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human MBP. No significant cross-reactivity or interference between Human MBP and analogues was observed.
• Sequence Length: 248

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for MBP elisa kit

Myelin basic protein (MBP) is a protein believed to be important in the process of myelination of nerves in the central nervous system (CNS). Myelin Basic Protein (MBP) is the major constituent of the CNS myelin synthesized by oligodendrocytes and Schwann cells. MBP, PLP (proteolipid proteins) and MOG (myelin oligodendrocyte glycoprotein) are potential primary encephalitogenic target antigens in multiple sclerosis.MBP was initially sequenced in 1979 after isolation from myelin membranes. Since that time, knockout mice deficient in MBP have been developed which showed decreased amounts of CNS myelination and a progressive disorder characterized by tremors, seizures, and early death. The gene for MBP is on chromosome 18; the protein localizes to the CNS and to various cells of the hematopoietic system.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MBP in samples. An antibody specific for MBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MBP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MBP bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 68509930
• NCBI GeneID: 4155
• NCBI Accession #: NP_001020252.1
• NCBI GenBank Nucleotide #: NM_001025081.1
• UniProt Accession #: P02686; Q15337; Q15338; Q15339; Q15340; A4FU54; A6NI84; A8MY86; A8MYL4; B3KY66; B7ZKS2; B7ZKS4
• Molecular Weight: 17,343 Da
• NCBI Official Full Name: myelin basic protein isoform 1
• NCBI Official Synonym Full Names: myelin basic protein
• NCBI Official Symbol: MBP
• NCBI Protein Information: myelin basic protein; Golli-MBP
• UniProt Protein Name: Myelin basic protein
• Protein Family: Myelin protein
• UniProt Gene Name: MBP
• UniProt Synonym Gene Names: MBP

NCBI Description

The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. [provided by RefSeq, Jul 2008]

Uniprot Description

The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.