DNA relaxase mbeA (mbeA) Recombinant Protein | mbeA recombinant protein
Recombinant Escherichia coli DNA relaxase mbeA (mbeA)
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
DNA relaxase mbeA (mbeA); Recombinant Escherichia coli DNA relaxase mbeA (mbeA); mbeA recombinant protein
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
1-517, Full length protein
Sequence
MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSFAEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLTLPDNLPETKQALAEGVTRGIDALYHAGEIKGRQDVIQALTEAGLEVVRVTRTSISIADPNGGKNIRLKGAFYEQSFADGRGVREKAERESRIYRENAEQRVQEARRICKRGCDIKRDENQRRYSPVHSLDRGIAGKTPGRGERGDDAAQEGRVKAGREYGHDVTGDSLSPVYREWRDALVSWREDTGEPGRNQEAGRDIAETEREDMGRGVCAGREQEIPCPSVREISGGDSLSGERVGTSEGVTQSDRAGNTFAERLRAAATGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQRELEIREERLIQEREHVLSLERERQPEIQERTLDGPSLGW
Sequence Length
517
Species
Escherichia coli
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
57,808 Da
NCBI Official Full Name
DNA relaxase MbeA
UniProt Protein Name
DNA relaxase MbeA
Protein Family
UniProt Gene Name
mbeA
Uniprot Description
Relaxase involved in plasmid ColE1 conjugative mobilization and is thus essential to promote the specific transfer of the plasmid during conjugation. First catalyzes the specific cleavage of one of the DNA strands at oriT, forming a covalent 5'-phosphotyrosine intermediate. The nic site corresponds to 5'-(1469)CTGG/CTTA(1462)-3' in the cleaved strand. The cleaved strand is then transferred through the dedicated type IV secretion apparatus. MbeA remains covalently linked at the 5' end of the strand, and once in the recipient cell, it probably catalyzes the rejoining of the two ends of the strand, re-forming the circular plasmid DNA. Is functional in vitro without a requirement for the conjugative accessory proteins.
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Product Notes
The mbeA mbea (Catalog #AAA1241023) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-517, Full length protein. The amino acid sequence is listed below: MIVKFHARGK GGGSGPVDYL LGRERNREGA TVLQGNPEEV RELIDATPFA KKYTSGVLSF AEKELPPGGR EKVMASFERV LMPGLEKNQY SILWVEHQDK GRLELNFVIP NMELQTGKRL QPYYDRADRP RIDAWQTLVN HHYGLHDPNA PENRRTLTLP DNLPETKQAL AEGVTRGIDA LYHAGEIKGR QDVIQALTEA GLEVVRVTRT SISIADPNGG KNIRLKGAFY EQSFADGRGV REKAERESRI YRENAEQRVQ EARRICKRGC DIKRDENQRR YSPVHSLDRG IAGKTPGRGE RGDDAAQEGR VKAGREYGHD VTGDSLSPVY REWRDALVSW REDTGEPGRN QEAGRDIAET EREDMGRGVC AGREQEIPCP SVREISGGDS LSGERVGTSE GVTQSDRAGN TFAERLRAAA TGLYAAAERM GERLRGIAED VFAYATGQRD AERAGHAVES AGAALERADR TLEPVIQREL EIREERLIQE REHVLSLERE RQPEIQERTL DGPSLGW. It is sometimes possible for the material contained within the vial of "DNA relaxase mbeA (mbeA), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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