Myoglobin/MB Recombinant Protein | MB recombinant protein

Recombinant Human Myoglobin/MB Protein (C-His)

Cat. Num. AAA2557195

• Gene Names: MB; PVALB; myoglobgin
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: Myoglobin/MB; Recombinant Human Myoglobin/MB Protein (C-His); MB; MGC13548; Myoglobin; PVALB; MB recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Supplied as a 0.2 mum filtered solution of 20mM Tris-HCl, 200mM NaCl, 1mM DTT, 20% glycerol, pH8.0.
• Sequence: Met1-Gly154
• Sequence Length: 154

• Species: Human
• Endotoxin: < 1.0 EU per mug as determined by the LAL method.
• Fusion Tag: C-His
• Preparation and Storage: Store at < -20 degree C, stable for 6 months. Please minimize freeze-thaw cycles.

Related Product Information for MB recombinant protein

Protein Construction: Recombinant Human Myoglobin is produced by our E Coli expression system and the target gene encoding Met1-Gly154 is expressed with a 6His tag at the C-terminus.

Background: Myoglobin is a 17KDa cytoplasmic oxygen-binding protein encoded by the MB gene and expressed in myocytes of the heart and skeletal muscle. Its name derives from its structural and functional similarity to hemoglobin, the oxygen binding protein found in red blood cells. Functions of myoglobin include oxygen storage and transport, as well as scavenging of NO and reactive oxygen species. Myoglobin also serves as a sensitive marker for muscle injury resulting from cardiac infarction. Myoglobin was the first protein to have its three-dimensional structure determined by X-ray crystallography.

NCBI and Uniprot Product Information

• NCBI GI #: 4885477
• NCBI GeneID: 4151
• NCBI Accession #: NP_005359.1
• NCBI GenBank Nucleotide #: NP_005359.1
• UniProt Accession #: P02144
• Molecular Weight: Molecular Mass: 18kDa; Actual Protein Molecular Mass: 18kDa
• NCBI Official Full Name: myoglobin
• NCBI Official Synonym Full Names: myoglobin
• NCBI Official Symbol: MB
• NCBI Official Synonym Symbols: PVALB; myoglobgin
• NCBI Protein Information: myoglobin
• UniProt Protein Name: Myoglobin
• Protein Family: Myoglobin
• UniProt Gene Name: MB
• UniProt Entry Name: MYG_HUMAN

NCBI Description

This gene encodes a member of the globin superfamily and is expressed in skeletal and cardiac muscles. The encoded protein is a haemoprotein contributing to intracellular oxygen storage and transcellular facilitated diffusion of oxygen. At least three alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

MB: Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. Belongs to the globin family.

Protein type: Carrier

Chromosomal Location of Human Ortholog: 22q13.1

Molecular Function: iron ion binding; heme binding; oxygen binding; oxygen transporter activity

Biological Process: slow-twitch skeletal muscle fiber contraction; response to hydrogen peroxide; oxygen transport; heart development; response to hormone stimulus; response to hypoxia; brown fat cell differentiation; enucleate erythrocyte differentiation