Human mannan-binding lectin serine peptidase 2 ELISA Kit | MASP2 elisa kit

Human mannan-binding lectin serine peptidase 2, MASP2 ELISA Kit

Cat. Num. AAA941977

• Gene Names: MASP2; sMAP; MAP19; MASP-2; MASP1P1
• Reactivity: Human
• Synonyms: mannan-binding lectin serine peptidase 2; Human mannan-binding lectin serine peptidase 2; MASP2 ELISA Kit; Human mannan-binding lectin serine peptidase 2 (MASP2) ELISA kit; MAP19; MASP-2; sMAP; MBL-associated plasma protein of 19 kD; MBL-associated serine protease 2; mannan-binding lectin serine protease 2; small MBL-associated protein; MASP2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human MASP2. No significant cross-reactivity or interference between human MASP2 and analogues was observed.
• Sequence Length: 686

• Samples: Serum, plasma, cell culture supernates, cerebrospinal fluid (csf)
• Assay Type: Quantitative Sandwich
• Detection Range: 23.5 pg/ml-1500 pg/ml
• Sensitivity: < 5.8 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MASP2 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MASP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MASP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MASP2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MASP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 21264363
• NCBI GeneID: 10747
• NCBI Accession #: NP_006601.2
• NCBI GenBank Nucleotide #: NM_006610.3
• UniProt Accession #: O00187; O75754; Q5TEQ5; Q5TER0; Q96QG4; Q9BZH0; Q9H498; Q9H499; Q9UBP3; Q9UC48; A8K458; A8MWJ2
• Molecular Weight: 75,702 Da
• NCBI Official Full Name: mannan-binding lectin serine protease 2 isoform 1 preproprotein
• NCBI Official Synonym Full Names: mannan-binding lectin serine peptidase 2
• NCBI Official Symbol: MASP2
• NCBI Official Synonym Symbols: sMAP; MAP19; MASP-2; MASP1P1
• NCBI Protein Information: mannan-binding lectin serine protease 2; small MBL-associated protein; MBL-associated serine protease 2; MBL-associated plasma protein of 19 kD; mannan-binding lectin serine protease 1 pseudogene 1; mannose-binding protein-associated serine protease 2; mannan-binding lectin serine peptidase 1 pseudogene 1
• UniProt Protein Name: Mannan-binding lectin serine protease 2
• Protein Family: Mannan-binding lectin serine protease
• UniProt Gene Name: MASP2
• UniProt Synonym Gene Names: MASP-2
• UniProt Entry Name: MASP2_HUMAN

NCBI Description

The Ra-reactive factor (RARF) is a complement-dependent bactericidal factor that binds to the Ra and R2 polysaccharides expressed by certain enterobacteria. Alternate splicing of this gene results in two transcript variants encoding two RARF components that are involved in the mannan-binding lectin pathway of complement activation. The longer isoform is cleaved into two chains which form a heterodimer linked by a disulfide bond. The encoded proteins are members of the trypsin family of peptidases. [provided by RefSeq, Jul 2008]

Uniprot Description

MASP2: Serum protease that plays an important role in the activation of the complement system via mannose-binding lectin. After activation by auto-catalytic cleavage it cleaves C2 and C4, leading to their activation and to the formation of C3 convertase. Defects in MASP2 are the cause of MASP2 deficiency (MASPD). MASPD is a disorder that results in autoimmune manifestations, recurrent severe infections, and chronic inflammatory disease. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.21.104; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p36.3-p36.2

Cellular Component: extracellular region

Molecular Function: peptidase activity; protein binding; serine-type endopeptidase activity; complement component C4b binding; calcium ion binding; calcium-dependent protein binding

Biological Process: innate immune response; complement activation, lectin pathway; proteolysis; complement activation, classical pathway; complement activation

Disease: Masp2 Deficiency