MAP kinase-activated protein kinase 5 (Mapkapk5) Recombinant Protein | Mapkapk5 recombinant protein

Recombinant Mouse MAP kinase-activated protein kinase 5 (Mapkapk5)

Cat. Num. AAA1230811

• Gene Names: Mapkapk5; MK5; PRAK
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: MAP kinase-activated protein kinase 5 (Mapkapk5); Recombinant Mouse MAP kinase-activated protein kinase 5 (Mapkapk5); Mapkapk5 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-473, Full length protein
• Sequence: MSEDSDMEKAIKETSILEEYSINWTQKLGAGISGPVRVCVKKSTQERFALKILLDRPKARNEVRLHMMCATHPNIVQIIEVFANSVQFPHESSPRARLLIVMEMMEGGELFHRISQHRHFTEKQASQVTKQIALALQHCHLLNIAHRDLKPENLLFKDNSLDAPVKLCDFGFAKVDQGDLMTPQFTPYYVAPQVLEAQRRHQKEKSGIIPTSPTPYTYNKSCDLWSLGVIIYVMLCGYPPFYSKHHSRTIPKDMRKKIMTGSFEFPEEEWSQISEMAKDVVRKLLKVKPEERLTIEGVLDHPWLNSTEALDNVLPSAQLMMDKAVVAGIQQAHAEQLANMRIQDLKVSLKPLHSVNNPILRKRKLLGTKPKDGIYIHDHENGTEDSNVALEKLRDVIAQCILPQAGKGENEDEKLNEVMQEAWKYNRECKLLRDALQSFSWNGRGFTDKVDRLKLAEVVKQVIEEQTLPHEPQ
• Sequence Length: 473

• Species: Mouse
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Related Product Information for Mapkapk5 recombinant protein

This protein is a member of the serine
threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1
ERK, MAPK14
p38-alpha, and MAPK11
p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported.

NCBI and Uniprot Product Information

• NCBI GI #: 6754636
• NCBI GeneID: 17165
• NCBI Accession #: NP_034895.1
• NCBI GenBank Nucleotide #: NM_010765.2
• UniProt Accession #: O54992; Q6QME4; Q6QME5; Q6QME6; Q6QME7; E9QQ45
• Molecular Weight: 53,967 Da
• NCBI Official Full Name: MAP kinase-activated protein kinase 5
• NCBI Official Synonym Full Names: MAP kinase-activated protein kinase 5
• NCBI Official Symbol: Mapkapk5
• NCBI Official Synonym Symbols: MK5; PRAK
• NCBI Protein Information: MAP kinase-activated protein kinase 5
• UniProt Protein Name: MAP kinase-activated protein kinase 5
• Protein Family: MAP kinase-activated protein kinase
• UniProt Gene Name: Mapkapk5
• UniProt Synonym Gene Names: MAPK-activated protein kinase 5; MAPKAP kinase 5; MAPKAPK-5

Uniprot Description

Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation. Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras-induced senescence and phosphorylating p53/TP53. Involved in post-transcriptional regulation of MYC by mediating phosphorylation of FOXO3: phosphorylation of FOXO3 leads to promote nuclear localization of FOXO3, enabling expression of miR-34b and miR-34c, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent MYC translation. Acts as a negative regulator of mTORC1 signaling by mediating phosphorylation and inhibition of RHEB. Part of the atypical MAPK signaling via its interaction with ERK3/MAPK6 or ERK4/MAPK4: the precise role of the complex formed with ERK3/MAPK6 or ERK4/MAPK4 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPK (ERK3/MAPK6 or ERK4/MAPK4), ERK3/MAPK6 (or ERK4/MAPK4) is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6 (or ERK4/MAPK4). Mediates phosphorylation of HSP27/HSPB1 in response to PKA/PRKACA stimulation, inducing F-actin rearrangement.