MAPKAPK3 recombinant protein

MAPKAPK3, Unactive recombinant protein

Cat. Num. AAA515410

• Gene Names: MAPKAPK3; 3PK; MK-3; MAPKAP3; MAPKAP-K3; MAPKAPK-3
• Applications: Western Blot
• Synonyms: MAPKAPK3; Unactive recombinant protein; 3PK; 3pK; MAPKAP3; MAPKAPK3 recombinant protein

• Host: E Coli
• Form/Format: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Sequence Length: 2553

• Applicable Applications for MAPKAPK3 recombinant protein: Kinase Assay, Western Blot (WB)
• Type: Recombinant Fusion Protein
• Species: Human
• Tag Information: GST tag
• Expression System: E.coli
• Source Note: Recombinant full-length human MAPKAPK3 was expressed in E. coli cells
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-PAGE

Related Product Information for MAPKAPK3 recombinant protein

Recombinant full-length human MAPKAPK3 was expressed in E. coli cells using an N-terminal GST tag.

Scientific Background: MAPKAPK3 is closely related to MAPKAPK2 sharing 72% nucleotide and 75% amino acid identity (1). MAPKAPK3 is activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of MAPKAPK3 as an integrative element of signaling in both mitogen and stress responses (2).

Product Categories/Family for MAPKAPK3 recombinant protein

Unactive Kinase; Signaling Proteins - Unactive Kinases

References

1. Sithanandam, G. et al: 3pK a new mitogen-activated protein kinase-activated protein kinase located in the small cell lung cancer tumor suppressor gene region. Molec. Cell. Biol. 16: 868-876, 1996.
2. Ludwig, S. et al: 3pK, a novel mitogen-activated protein (MAP) kinase-activated protein kinase, is targeted by three MAP kinase pathways. Molec. Cell. Biol. 16: 6687-6697, 1996.

NCBI and Uniprot Product Information

• NCBI GI #: 345441754
• NCBI GeneID: 7867
• NCBI Accession #: NP_004626.1
• NCBI GenBank Nucleotide #: NM_004635
• UniProt Accession #: Q16644; B5BU67
• Molecular Weight: ~69 kDa
• NCBI Official Full Name: Homo sapiens mitogen-activated protein kinase-activated protein kinase 3 (MAPKAPK3), transcript variant 3, mRNA
• NCBI Official Synonym Full Names: mitogen-activated protein kinase-activated protein kinase 3
• NCBI Official Symbol: MAPKAPK3
• NCBI Official Synonym Symbols: 3PK; MK-3; MAPKAP3; MAPKAP-K3; MAPKAPK-3
• NCBI Protein Information: MAP kinase-activated protein kinase 3; MAPKAP kinase 3; chromosome 3p kinase; MAPK-activated protein kinase 3
• UniProt Protein Name: MAP kinase-activated protein kinase 3
• Protein Family: MAP kinase-activated protein kinase
• UniProt Gene Name: MAPKAPK3
• UniProt Synonym Gene Names: MAPK-activated protein kinase 3; MAPKAP kinase 3; MAPKAP-K3; MAPKAPK-3; MK-3; 3pK
• UniProt Entry Name: MAPK3_HUMAN

NCBI Description

This gene encodes a member of the Ser/Thr protein kinase family. This kinase functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011]

Uniprot Description

Function: Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, cell migration, chromatin remodeling and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. MAPKAPK2 and MAPKAPK3, share the same function and substrate specificity, but MAPKAPK3 kinase activity and level in protein expression are lower compared to MAPKAPK2. Phosphorylates HSP27/HSPB1, KRT18, KRT20, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins, such as TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3. Also acts as a modulator of Polycomb-mediated repression. Ref.1 Ref.7 Ref.8 Ref.11 Ref.12 Ref.14

Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Ref.7

Enzyme regulation: Activated following phosphorylation by p38-alpha/MAPK14 following various stresses. Inhibited by ligand 5B (2'-[2-(1,3-benzodioxol-5-yl)pyrimidin-4-yl]-5',6'-dihydrospiro[piperidine-4,7'-pyrrolo[3,2-c]pyridin]- 4'(1'h)-one) and ligand P4O (2-[2-(2-fluorophenyl)pyridin-4-yl]-1,5,6,7-tetrahydro- 4h-pyrrolo[3,2-c]pyridin-4-one), 2 ATP-competitive inhibitors.

Subunit structure: Heterodimer with p38-alpha/MAPK14. The heterodimer with p38-alpha/MAPK14 forms a stable complex: molecules are positioned 'face to face' so that the ATP-binding sites of both kinases are at the heterodimer interface

By similarity. Interacts with TCF3 and with polycomb proteins, such as PCH2 and BMI1/PCGF4. Ref.9 Ref.11

Subcellular location: Nucleus. Cytoplasm. Note: Predominantly located in the nucleus, when activated it translocates to the cytoplasm. Ref.10

Tissue specificity: Widely expressed, with a higher expression level observed in heart and skeletal muscle. No expression in brain. Ref.1 Ref.2

Post-translational modification: Phosphorylated and activated by MAPK1/ERK2 and MAPK3/ERK1. Phosphorylated and activated by MAP kinase p38-alpha/MAPK14 at Thr-201, Ser-251 and Thr-313

By similarity. Ref.1 Ref.2

Sequence similarities: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.Contains 1 protein kinase domain.