Rat Mitogen Activated Protein Kinase -Activa Protein Kinase 3 ELISA Kit | MAPKAPK3 elisa kit

Rat Mitogen Activated Protein Kinase -Activa Protein Kinase 3 ELISA Kit

Cat. Num. AAA730741

• Gene Names: MAPKAPK3; 3PK; MK-3; MAPKAP3; MAPKAP-K3; MAPKAPK-3
• Reactivity: Rat
• Synonyms: Mitogen Activated Protein Kinase -Activa Protein Kinase 3; Rat Mitogen Activated Protein Kinase -Activa Protein Kinase 3 ELISA Kit; MAPKAPK3 elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This MAPK3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat MAPK3. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MAPKAPK3 elisa kit

Principle of the assay: MAPK3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MAPK3 antibody and an MAPK3-HRP conjugate. The assay sample and buffer are incubated together with MAPK3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MAPK3 concentration since MAPK3 from samples and MAPK3-HRP conjugate compete for the anti-MAPK3 antibody binding site. Since the number of sites is limited, as more sites are occupied by MAPK3 from the sample, fewer sites are left to bind MAPK3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MAPK3 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4758700
• NCBI GeneID: 7867
• NCBI Accession #: NP_004626.1
• NCBI GenBank Nucleotide #: NM_004635.4
• Molecular Weight: 42,987 Da
• NCBI Official Full Name: MAP kinase-activated protein kinase 3
• NCBI Official Synonym Full Names: mitogen-activated protein kinase-activated protein kinase 3
• NCBI Official Symbol: MAPKAPK3
• NCBI Official Synonym Symbols: 3PK; MK-3; MAPKAP3; MAPKAP-K3; MAPKAPK-3
• NCBI Protein Information: MAP kinase-activated protein kinase 3; MAPKAP kinase 3; chromosome 3p kinase; MAPK-activated protein kinase 3
• UniProt Protein Name: MAP kinase-activated protein kinase 3
• Protein Family: MAP kinase-activated protein kinase
• UniProt Gene Name: MAPKAPK3
• UniProt Synonym Gene Names: MAPK-activated protein kinase 3; MAPKAP kinase 3; MAPKAP-K3; MAPKAPK-3; MK-3; 3pK
• UniProt Entry Name: MAPK3_HUMAN

NCBI Description

This gene encodes a member of the Ser/Thr protein kinase family. This kinase functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011]

Uniprot Description

MAPKAPK3: a member of the MAPKAPK family of protein kinases. Activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation.

Protein type: Protein kinase, CAMK; EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CAMK group; MAPKAPK family; MAPKAPK subfamily

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: nucleoplasm; nuclear membrane; cytoplasm; cytosol; nucleus

Molecular Function: MAP kinase kinase activity; protein serine/threonine kinase activity; protein binding; ATP binding

Biological Process: nerve growth factor receptor signaling pathway; activation of MAPK activity; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; response to lipopolysaccharide; toll-like receptor 3 signaling pathway; signal transduction; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; peptidyl-serine phosphorylation; response to cytokine stimulus; Ras protein signal transduction; toll-like receptor signaling pathway; response to stress; innate immune response; toll-like receptor 9 signaling pathway; vascular endothelial growth factor receptor signaling pathway; toll-like receptor 4 signaling pathway