Human Mitogen-activated protein kinase 8 ELISA Kit | MAPK8 elisa kit

Human Mitogen-activated protein kinase 8 ELISA Kit

Cat. Num. AAA763420

• Gene Names: Mapk8; JNK
• Reactivity: Human
• Synonyms: Mitogen-activated protein kinase 8; Human Mitogen-activated protein kinase 8 ELISA Kit; MAPK8/PRKM8/SAPK1/c-Jun N-terminal kinase 1/JNK1 alpha protein kinase/JNK1 beta protein kinase/JNK1JNK1A2/JNK-46/JUN N-terminal kinase/MAP kinase 8/MAPK 8/mitogen-activated protein kinase 8/mitogen-activated protein kinase 8 isoform JNK1 alpha1/mitogen-activated protein kinase 8 isoform JNK1 beta2/PRKM8JNK/protein kinase JNK1/SAPK1JNK21B1; 2/Stress-activated protein kinase 1/Stress-activated protein kinase JNK1; MAPK8 elisa kit

• Reactivity: Human
• Sequence Length: 427

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for MAPK8 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human MAPK8 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human MAPK8 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human MAPK8 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human MAPK8 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 694879445
• NCBI GeneID: 116554
• NCBI Accession #: NP_446281.2
• NCBI GenBank Nucleotide #: NM_053829.2
• Molecular Weight: 46,807 Da
• NCBI Official Full Name: mitogen-activated protein kinase 8
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 8
• NCBI Official Symbol: Mapk8
• NCBI Official Synonym Symbols: JNK
• NCBI Protein Information: mitogen-activated protein kinase 8
• UniProt Protein Name: Mitogen-activated protein kinase 8
• Protein Family: Mitogen-activated protein kinase
• UniProt Gene Name: Mapk8
• UniProt Synonym Gene Names: Jnk1; Prkm8; MAP kinase 8; MAPK 8
• UniProt Entry Name: MK08_RAT

NCBI Description

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. [provided by RefSeq, Jul 2012]

Uniprot Description

JNK1: a protein kinase of the MAPK family that is potently activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, pro-inflammatory cytokines and, in some instances, by growth factors and GPCR agonists. Substrates include a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2, thus regulating AP-1 transcriptional activity. In T- cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. Binds to at least four scaffolding proteins, JIP-1, -2, -3 and -4. Activity increased in obesity. Inhibition or mouse knockout increases insulin sensitivity. Part of NFB pathway involved in inflammation and cancer, and signals downstream of Ras, though possibly as an apoptotic negative regulator of growth. Four alternatively-spliced isoforms have been described.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, CMGC; EC 2.7.11.24; CMGC group; MAPK family; JNK subfamily; MAPK/JNK subfamily

Cellular Component: axon; cytoplasm; cytosol; dendrite cytoplasm; intracellular; mitochondrion; nucleus; perikaryon; podosome; vesicle

Molecular Function: ATP binding; enzyme binding; histone deacetylase binding; histone deacetylase regulator activity; JUN kinase activity; kinase activity; kinesin binding; MAP kinase activity; protein binding; protein serine/threonine kinase activity

Biological Process: dendrite morphogenesis; inflammatory response; JNK cascade; JUN phosphorylation; MAPKKK cascade; negative regulation of apoptosis; negative regulation of protein binding; neurite development; neuron development; neuron migration; ossification; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; positive regulation of apoptosis; positive regulation of cell migration; positive regulation of cyclase activity; positive regulation of DNA replication; positive regulation of microtubule polymerization; positive regulation of neuron apoptosis; positive regulation of protein metabolic process; protein amino acid phosphorylation; regulation of circadian rhythm; regulation of gene expression; regulation of histone deacetylation; regulation of protein localization; regulation of transcription, DNA-dependent; response to cadmium ion; response to heat; response to hydrogen peroxide; response to osmotic stress; response to steroid hormone stimulus; response to UV; rhythmic process; signal transduction