Mitogen Activated Protein Kinase 13 Recombinant Protein | MAPK13 recombinant protein

Recombinant Mitogen Activated Protein Kinase 13 (MAPK13)

Cat. Num. AAA2033233

• Gene Names: MAPK13; SAPK4; PRKM13; MAPK 13; MAPK-13; p38delta
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Mitogen Activated Protein Kinase 13; Recombinant Mitogen Activated Protein Kinase 13 (MAPK13); SAPK4; p38 Delta; Mitogen-activated protein kinase p38 delta; Stress-activated protein kinase 4; MAPK13 recombinant protein

• Host: E Coli
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: MSLIRKKGFY KQDVNKTAWE LPKTYVSPTH VGSGAYGSVC SAIDKRSGEK VAIKKLSRPF QSEIFAKRAY RELLLLKHMQ HENVIGLLDV FTPASSLRNF YDFYLVMPFM QTDLQKIMGM EFSEEKIQYL VYQMLKGLKY IHSAGVVHRD LKPGNLAVNE DCELKILDFG LARHADAEMT GYVVTRWYRA PEVILSWMHY NQTVDIWSVG CIMAEMLTGK TLFKGKDYLD QLTQILKVTG VPGTEFVQKL NDKAAKSYIQ SLPQTPRKDF TQLFPRASPQ AADLLEKMLE LDVDKRLTAA QALTHPFFEP FRDPEEETEA QQPFDDSLEH EKLTVDEWKQ HIYKEIVNFS PIARKDSRRRSGMKL
• Sequence Length: 365

• Applicable Applications for MAPK13 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 45.8kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Met1~Leu365
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4506085
• NCBI GeneID: 5603
• NCBI Accession #: NP_002745.1
• NCBI GenBank Nucleotide #: NM_002754.4
• Molecular Weight: 28,779 Da
• NCBI Official Full Name: mitogen-activated protein kinase 13
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 13
• NCBI Official Symbol: MAPK13
• NCBI Official Synonym Symbols: SAPK4; PRKM13; MAPK 13; MAPK-13; p38delta
• NCBI Protein Information: mitogen-activated protein kinase 13
• UniProt Protein Name: Mitogen-activated protein kinase 13
• UniProt Gene Name: MAPK13
• UniProt Synonym Gene Names: PRKM13; SAPK4; MAP kinase 13; MAPK 13; MAP kinase p38 delta
• UniProt Entry Name: MK13_HUMAN

NCBI Description

This gene encodes a member of the mitogen-activated protein (MAP) kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The encoded protein is a p38 MAP kinase and is activated by proinflammatory cytokines and cellular stress. Substrates of the encoded protein include the transcription factor ATF2 and the microtubule dynamics regulator stathmin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

P38D: a proline-directed ser/thr MAP kinase, and one of four p38 kinases that play important roles in cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have a few hundred substrates each. MAPK13 is one of the less studied p38 MAPK isoforms. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets. Plays a role in the regulation of protein translation by phosphorylating and inactivating EEF2K. Involved in cytoskeletal remodeling through phosphorylation of tau and STH. Mediates UV irradiation induced up-regulation of the gene expression of CXCL14. Plays an important role in the regulation of epidermal keratinocyte differentiation, apoptosis and skin tumor development. Phosphorylates the transcriptional activator Myb in response to stress which leads to rapid Myb degradation via a proteasome-dependent pathway. Phosphorylates and down-regulates PKD1 during regulation of insulin secretion in pancreatic beta cells. Expressed in testes, pancreas, small intestine, lung and kidney. Abundant in macrophages, also present in neutrophils, CD4+ T-cells, and endothelial cells. This description may include information from UniProtKB

Protein type: EC 2.7.11.24; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, CMGC; CMGC group; MAPK family; MAPK/p38 subfamily; p38 subfamily

Chromosomal Location of Human Ortholog: 6p21.31

Cellular Component: cytosol

Molecular Function: ATP binding; MAP kinase activity; protein binding; protein serine/threonine kinase activity

Biological Process: cell cycle; MAPKKK cascade; nerve growth factor receptor signaling pathway; peptidyl-serine phosphorylation; positive regulation of inflammatory response; positive regulation of interleukin-6 production; Ras protein signal transduction; regulation of transcription, DNA-dependent; response to osmotic stress; response to stress; transcription, DNA-dependent; vascular endothelial growth factor receptor signaling pathway