Mitogen Activated Protein Kinase Kinase 7 (MAP2K7) Recombinant Protein | MAP2K7 recombinant protein

Recombinant Mitogen Activated Protein Kinase Kinase 7 (MAP2K7)

Cat. Num. AAA2011456

• Gene Names: Map2k7; Mek7; Mkk7; sek2; Jnkk2; MEK 7; JNKK 2; Mapkk7; Prkmk7; MAPKK 7; 5930412N11Rik
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Mitogen Activated Protein Kinase Kinase 7 (MAP2K7); Recombinant Mitogen Activated Protein Kinase Kinase 7 (MAP2K7); MAP2K7 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-MDLDVVLKSH DCPYIVQCFG TFITNTDVFI AMELMGTCAE KLKKRMQGPI PERILGKMTV AIVKALYYLK EKHGVIHRDV KPSNILLDER GQIKLCDFGI SGRLVDSKAK TRSAGCAAYM APERIDPPDP TKPDYDIRAD VWSLGISLVE LATGQFPYKN CKTDFEVLTK VLQEEPPLLP GHMGFSGDFQ SFVKDCLTKD HRKRPKYNKL LEHSFIKHYE ILEVDVASWF KDVMAKTESP RTSGVLSQH
• Sequence Length: 468

• Applicable Applications for MAP2K7 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Met181~His429 (Accession # Q8CE90) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 110347549
• NCBI GeneID: 26400
• NCBI Accession #: NP_001036022.1
• NCBI GenBank Nucleotide #: NM_001042557.2
• UniProt Accession #: Q8CE90; O35406; O35720; O35871; O35872; O54780; O70242; O70243; Q8BSP1; Q9QWG6; Q9R1Z3; Q9R1Z4
• Molecular Weight: 32.0kDa
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 7 isoform 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 7
• NCBI Official Symbol: Map2k7
• NCBI Official Synonym Symbols: Mek7; Mkk7; sek2; Jnkk2; MEK 7; JNKK 2; Mapkk7; Prkmk7; MAPKK 7; 5930412N11Rik
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 7; JNK kinase 2; MAPK/ERK kinase 7; MAP kinase kinase 7; JNK-activating kinase 2; c-Jun N-terminal kinase kinase 2; mitogen activated protein kinase kinase 7; protein kinase, mitogen activated, kinase 7
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 7
• Protein Family: Dual specificity mitogen-activated protein kinase kinase
• UniProt Gene Name: Map2k7
• UniProt Synonym Gene Names: Mkk7; MAP kinase kinase 7; MAPKK 7; MEK 7; JNK kinase 2; JNKK 2
• UniProt Entry Name: MP2K7_MOUSE

Uniprot Description

MKK7: a dual-specificity protein kinase of the STE7 family. Activates JNK1 and -2 by phosphorylating a Thr and a Tyr residue in the activation loop. Is activated by proinflammatory cytokines and environmental stress. Three alternatively spliced isoforms have been reported.

Protein type: Kinase, protein; Protein kinase, STE; Protein kinase, dual-specificity (non-receptor); EC 2.7.12.2; STE group; STE7 family

Cellular Component: cytoplasm; nucleus; cytosol

Molecular Function: protein C-terminus binding; metal ion binding; magnesium ion binding; nucleotide binding; mitogen-activated protein kinase kinase kinase binding; JUN kinase kinase activity; protein phosphatase binding; protein kinase binding; protein kinase activity; MAP kinase kinase activity; transferase activity; protein serine/threonine kinase activity; protein binding; enzyme binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; kinase activity; ATP binding

Biological Process: apoptosis; response to heat; positive regulation of neuron apoptosis; stress-activated MAPK cascade; response to stress; JNK cascade; positive regulation of protein amino acid phosphorylation; response to osmotic stress; protein amino acid phosphorylation; phosphorylation; activation of JNK activity; response to UV