Dual specificity mitogen-activated protein kinase kinase 4 (MAP2K4) Recombinant Protein | MAP2K4 recombinant protein

Recombinant Human Dual specificity mitogen-activated protein kinase kinase 4 (MAP2K4)

Cat. Num. AAA952063

• Gene Names: MAP2K4; JNKK; MEK4; MKK4; SEK1; SKK1; JNKK1; SERK1; MAPKK4; PRKMK4; SAPKK1; SAPKK-1
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Dual specificity mitogen-activated protein kinase kinase 4 (MAP2K4); Recombinant Human Dual specificity mitogen-activated protein kinase kinase 4 (MAP2K4); MAP2K4 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 2-399, Full length protein
• Sequence: AAPSPSGGGGSGGGSGSGTPGPVGSPAPGHPAVSSMQGKRKALKLNFANPPFKSTARFTLNPNPTGVQNPHIERLRTHSIESSGKLKISPEQHWDFTAEDLKDLGEIGRGAYGSVNKMVHKPSGQIMAVKRIRSTVDEKEQKQLLMDLDVVMRSSDCPYIVQFYGALFREGDCWICMELMSTSFDKFYKYVYSVLDDVIPEEILGKITLATVKALNHLKENLKIIHRDIKPSNILLDRSGNIKLCDFGISGQLVDSIAKTRDAGCRPYMAPERIDPSASRQGYDVRSDVWSLGITLYELATGRFPYPKWNSVFDQLTQVVKGDPPQLSNSEEREFSPSFINFVNLCLTKDESKRPKYKELLKHPFILMYEERAVEVACYVCKILDQMPATPSSPMYVD
• Sequence Length: 398

• Species: Human
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Related Product Information for MAP2K4 recombinant protein

This gene encodes a dual specificity protein kinase that belongs to the Ser
Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8
JNK1, MAPK9
JNK2, and MAPK14
p38, but not MAPK1
ERK2 or MAPK3
ERK3. This kinase is phosphorylated, and thus activated by MAP3K1
MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver.

NCBI and Uniprot Product Information

• NCBI GI #: 526479851
• NCBI GeneID: 6416
• NCBI Accession #: NP_001268364.1
• NCBI GenBank Nucleotide #: NM_001281435.1
• UniProt Accession #: P45985; Q5U0B8; Q6FHX4; Q6P9H2; Q6PIE6; B2R7N7; B3KYB2; D3DTS5
• Molecular Weight: 45,584 Da
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 4 isoform 2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 4
• NCBI Official Symbol: MAP2K4
• NCBI Official Synonym Symbols: JNKK; MEK4; MKK4; SEK1; SKK1; JNKK1; SERK1; MAPKK4; PRKMK4; SAPKK1; SAPKK-1
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 4
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 4
• Protein Family: Dual specificity mitogen-activated protein kinase kinase
• UniProt Gene Name: MAP2K4
• UniProt Synonym Gene Names: JNKK1; MEK4; MKK4; PRKMK4; SEK1; SERK1; SKK1; MAP kinase kinase 4; MAPKK 4; MEK 4; SEK1; SAPK kinase 1; SAPKK-1; SAPKK1; JNKK

NCBI Description

This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to proinflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14.