Canine Microtubule Associated Protein 1 Light Chain 3 Alpha ELISA Kit | MAP1LC3alpha elisa kit

Canine Microtubule Associated Protein 1 Light Chain 3 Alpha ELISA Kit

Cat. Num. AAA90211

• Gene Names: MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
• Reactivity: Canine
• Synonyms: Microtubule Associated Protein 1 Light Chain 3 Alpha; Canine Microtubule Associated Protein 1 Light Chain 3 Alpha ELISA Kit; MAP1LC3alpha elisa kit

• Reactivity: Canine
• Specificity: No significant cross-reactivity or interference between this analyte and analogues is observed.
• Sequence Length: 121

• Assay Type: Quantitative Sandwich
• Detection Range: 3.12 ng/ml - 100 ng/ml.
• Sensitivity: 1.0 ng/ml.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for MAP1LC3alpha elisa kit

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! This kit is intended to be used for determination the level of MAP1LC3A (hereafter termed "analyte") in undiluted original Canine body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

NCBI and Uniprot Product Information

• NCBI GI #: 85701362
• NCBI GeneID: 84557
• UniProt Accession #: Q9H492; Q9BXW5; E1P5P4; E1P5P5
• Molecular Weight: 14,272 Da
• NCBI Official Full Name: Microtubule-associated proteins 1A/1B light chain 3A
• NCBI Official Synonym Full Names: microtubule-associated protein 1 light chain 3 alpha
• NCBI Official Symbol: MAP1LC3A
• NCBI Official Synonym Symbols: LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
• NCBI Protein Information: microtubule-associated proteins 1A/1B light chain 3A; MAP1A/MAP1B LC3 A; MAP1A/1B light chain 3 A; MAP1A/MAP1B light chain 3 A; MAP1 light chain 3-like protein 1; autophagy-related ubiquitin-like modifier LC3 A
• UniProt Protein Name: Microtubule-associated proteins 1A/1B light chain 3A
• UniProt Gene Name: MAP1LC3A
• UniProt Synonym Gene Names: MAP1A/MAP1B LC3 A
• UniProt Entry Name: MLP3A_HUMAN

NCBI Description

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis. [provided by RefSeq, Feb 2012]

Uniprot Description

LC3A: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin-like modifier; Autophagy; Microtubule-binding; Vesicle

Chromosomal Location of Human Ortholog: 20q11.22

Cellular Component: microtubule; extrinsic to membrane; late endosome; autophagic vacuole; cytoplasmic vesicle; cytosol

Molecular Function: protein binding; phosphatidylethanolamine binding; microtubule binding; GABA receptor binding; phospholipid binding

Biological Process: mitochondrion degradation; cellular response to nitrogen starvation; autophagic vacuole formation