Rat malonyl coenzyme A ELISA Kit | malonyl CoA elisa kit

Rat malonyl coenzyme A ELISA Kit

Cat. Num. AAA701511

• Gene Names: MLYCD; MCD
• Reactivity: Rat
• Synonyms: malonyl coenzyme A; Rat malonyl coenzyme A ELISA Kit; Rat malonyl coenzyme A ELISA kit; malonyl CoA elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of rat malonyl CoA. No significant cross-reactivity or interference between rat malonyl CoA and analogues was observed.
• Sequence Length: 493

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Competitive
• Detection Range: 1.25 ng/ml-20 ng/ml
• Sensitivity: < 0.625 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for malonyl CoA elisa kit

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to malonyl CoA. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated malonyl CoA. The competitive inhibition reaction is launched between with HRP-conjugated malonyl CoA and malonyl CoA in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of malonyl CoA in the sample. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 110349750
• NCBI GeneID: 23417
• NCBI Accession #: NP_036345.2
• NCBI GenBank Nucleotide #: NM_012213.2
• UniProt Accession #: O95822; Q9UNU5; Q9Y3F2
• Molecular Weight: 50,946 Da
• NCBI Official Full Name: malonyl-CoA decarboxylase, mitochondrial
• NCBI Official Synonym Full Names: malonyl-CoA decarboxylase
• NCBI Official Symbol: MLYCD
• NCBI Official Synonym Symbols: MCD
• NCBI Protein Information: malonyl-CoA decarboxylase, mitochondrial; malonyl coenzyme A decarboxylase
• UniProt Protein Name: Malonyl-CoA decarboxylase, mitochondrial
• UniProt Gene Name: MLYCD
• UniProt Synonym Gene Names: MCD
• UniProt Entry Name: DCMC_HUMAN

NCBI Description

The product of this gene catalyzes the breakdown of malonyl-CoA to acetyl-CoA and carbon dioxide. Malonyl-CoA is an intermediate in fatty acid biosynthesis, and also inhibits the transport of fatty acyl CoAs into mitochondria. Consequently, the encoded protein acts to increase the rate of fatty acid oxidation. It is found in mitochondria, peroxisomes, and the cytoplasm. Mutations in this gene result in malonyl-CoA decarboyxlase deficiency. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Catalyzes the conversion of malonyl-CoA to acetyl-CoA. In the fatty acid biosynthesis MCD selectively removes malonyl-CoA and thus assures that methyl-malonyl-CoA is the only chain elongating substrate for fatty acid synthase and that fatty acids with multiple methyl side chains are produced. In peroxisomes it may be involved in degrading intraperoxisomal malonyl-CoA, which is generated by the peroxisomal beta-oxidation of odd chain-length dicarboxylic fatty acids. Plays a role in the metabolic balance between glucose and lipid oxidation in muscle independent of alterations in insulin signaling. May play a role in controlling the extent of ischemic injury by promoting glucose oxidation.4 PublicationsManual assertion based on experiment in:Ref.2

Catalytic activity: Malonyl-CoA = acetyl-CoA + CO2.5 PublicationsManual assertion based on experiment in:Ref.1

Enzyme regulation: Malonyl-CoA decarboxylase activity does not require any cofactors or divalent metal ions. Formation of interchain disulfide bonds leads to positive cooperativity between active sites and increases the affinity for malonyl-CoA and the catalytic efficiency (in vitro).2 PublicationsManual assertion based on experiment in:Ref.6