Human myelin associated glycoprotein ELISA Kit | MAG elisa kit

Human myelin-associated glycoprotein, MAG ELISA Kit

Cat. Num. AAA907002

• Gene Names: MAG; GMA; S-MAG; SIGLEC4A; SIGLEC-4A
• Reactivity: Human
• Synonyms: myelin associated glycoprotein; Human myelin-associated glycoprotein; MAG ELISA Kit; Human myelin-associated glycoprotein (MAG) ELISA Kit; GMA; S-MAG; SIGLEC-4A; SIGLEC4A; myelin-associated glycoprotein; sialic acid-binding immunoglobulin-like lectin 4A; MAG elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human MAG. No significant cross-reactivity or interference between Human MAG and analogues was observed.
• Sequence Length: 601

• Samples: Serum, plasma, cerebrospinal fluid (CSF)
• Assay Type: Sandwich
• Detection Range: 0.625 ng/ml-40 ng/ml
• Sensitivity: 0.156 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Cell Adhesion
• Protein Biological Process 3: Cell adhesion
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MAG elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MAG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MAG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAG bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 312836851
• NCBI GeneID: 4099
• NCBI Accession #: NP_001186145.1
• NCBI GenBank Nucleotide #: NM_001199216.1
• UniProt Accession #: P20916; Q567S4; B7Z2E5; F5GYC0
• Molecular Weight: 69,069 Da
• NCBI Official Full Name: myelin-associated glycoprotein isoform c
• NCBI Official Synonym Full Names: myelin associated glycoprotein
• NCBI Official Symbol: MAG
• NCBI Official Synonym Symbols: GMA; S-MAG; SIGLEC4A; SIGLEC-4A
• NCBI Protein Information: myelin-associated glycoprotein; sialic acid binding Ig-like lectin 4A; sialic acid-binding immunoglobulin-like lectin 4A
• UniProt Protein Name: Myelin-associated glycoprotein
• Protein Family: Myelin-associated glycoprotein
• UniProt Gene Name: MAG
• UniProt Synonym Gene Names: GMA
• UniProt Entry Name: MAG_HUMAN

NCBI Description

The protein encoded by this gene is a type I membrane protein and member of the immunoglobulin superfamily. It is thought to be involved in the process of myelination. It is a lectin that binds to sialylated glycoconjugates and mediates certain myelin-neuron cell-cell interactions. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Nov 2010]

Uniprot Description

MAG: Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3- linked sialic acid. Binds to RTN4R. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: paranode region of axon; integral to membrane; plasma membrane

Molecular Function: carbohydrate binding

Biological Process: substantia nigra development; nerve growth factor receptor signaling pathway; negative regulation of axonogenesis; regulation of axonogenesis; cell adhesion; blood coagulation; leukocyte migration