MAD2L1 recombinant protein

MAD2L1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121225

• Gene Names: MAD2L1; MAD2; HSMAD2
• Purity: >96% as determined by SDS-PAGE
• Synonyms: MAD2L1; MAD2L1 Protein; Human; Recombinant (His Tag); Human MAD2L1/MAD2 Protein (His Tag); HSMAD2 Protein; MAD2 Protein; MAD2 mitotic arrest deficient-like 1 (yeast); MAD2L1 recombinant protein

• Host: E Coli
• Purity/Purification: >96% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, 20% glycerol, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asp205

• Species: Human
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human MAD2L1 (Q13257)(Met 1-Asp 205) was expressed, with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for MAD2L1 recombinant protein

Background: Mitotic spindle assembly checkpoint protein MAD2A, also known as HsMAD2, Mitotic arrest deficient 2-like protein 1, MAD2-like protein 1, MAD2L1, and MAD2, is a nucleus and cytoplasm protein that belongs to the MAD2 family. MAD2L1 is a component of the spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase-promoting complex by sequestering CDC2 until all chromosomes are aligned at the metaphase plate. MAD2L1 has two highly different native conformations, an inactive open conformation that cannot bind CDC2 and that predominates in cytosolic monomers, and an active closed conformation. MAD2L1 in the closed conformation preferentially dimerizes with another molecule in the open conformation, but can also form a dimer with a molecule in the closed conformation. Formation of a heterotetrameric core complex containing two molecules of MAD1L1 and MAD2L1 in the closed conformation promotes binding of another molecule of MAD2L1 in the open conformation and the conversion of the open to the closed-form and thereby promotes interaction with CDC2.

References

Luo X., et al., 2000, Nat. Struct. Biol. 7:224-229. Sironi L., et al., 2002, EMBO J. 21:2496-2506. Mapelli M., et al., 2007, Cell 131:730-743. Ho C.-Y., et al., 2008, J. Cell. Biochem. 105:835-846. Skinner J.J., et al., 2008, J. Cell Biol. 183:761-768.

NCBI and Uniprot Product Information

• NCBI GI #: 4505067
• NCBI GeneID: 4085
• NCBI Accession #: NP_002349.1
• NCBI GenBank Nucleotide #: NM_002358.3
• UniProt Accession #: Q13257; Q53F56; Q548X9; Q6IRW7
• Molecular Weight: 23,510 Da
• NCBI Official Full Name: mitotic spindle assembly checkpoint protein MAD2A
• NCBI Official Synonym Full Names: MAD2 mitotic arrest deficient-like 1 (yeast)
• NCBI Official Symbol: MAD2L1
• NCBI Official Synonym Symbols: MAD2; HSMAD2
• NCBI Protein Information: mitotic spindle assembly checkpoint protein MAD2A; MAD2-like protein 1; mitotic arrest deficient 2-like protein 1; mitotic arrest deficient, yeast, homolog-like 1; MAD2 (mitotic arrest deficient, yeast, homolog)-like 1
• UniProt Protein Name: Mitotic spindle assembly checkpoint protein MAD2A
• Protein Family: MAD2L1-binding protein
• UniProt Gene Name: MAD2L1
• UniProt Synonym Gene Names: MAD2; HsMAD2
• UniProt Entry Name: MD2L1_HUMAN

NCBI Description

MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq, Jul 2008]

Uniprot Description

MAD2L1: required for the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and delays the onset of anaphase when this process is not complete. Inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. Interacts with Cdc20, MAD2L1BP and with ADAM17/TACE

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 4q27

Cellular Component: kinetochore; spindle pole; perinuclear region of cytoplasm; nuclear pore; nucleus; cytosol

Molecular Function: identical protein binding; protein binding; protein homodimerization activity

Biological Process: negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitotic sister chromatid segregation; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle checkpoint; mitotic cell cycle spindle assembly checkpoint; negative regulation of mitotic cell cycle; mitotic cell cycle; negative regulation of protein catabolic process; negative regulation of apoptosis