Macrolide export ATP-binding/permease protein MacB (macB) Recombinant Protein | macB recombinant protein

Recombinant Escherichia coli Macrolide export ATP-binding/permease protein MacB (macB)

• Gene Names: macB; ECK0870; JW0863; ybjZ
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Macrolide export ATP-binding/permease protein MacB (macB); Recombinant Escherichia coli Macrolide export ATP-binding/permease protein MacB (macB); Recombinant Macrolide export ATP-binding/permease protein MacB (macB); Macrolide export ATP-binding/permease protein MacB EC= 3.6.3.-; macB recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-648
• Sequence: MTPLLELKDIRRSYPAGDEQVEVLKGISLDIYAGEMVAIVGASGSGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLTAEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALMNGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRDGEIVRNPPAIEKVNVTGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTMLGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYDDLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQEQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVLRVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMDGVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARASDVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVTGILFGWLPARNAARLDPVDALARE
• Sequence Length: 648

• Species: Escherichia coli (strain K12)
• Preparation and Storage: Store at -20 degree C. For extended storage, store at -20 or -80 degree C.

NCBI and Uniprot Product Information

• NCBI GI #: 16128847
• NCBI GeneID: 945164
• NCBI Accession #: NP_415400.1
• NCBI GenBank Nucleotide #: NC_000913.2
• UniProt Accession #: P75831
• Molecular Weight: 70,702 Da
• NCBI Official Full Name: fused macrolide transporter subunits of ABC superfamily: ATP-binding component/membrane component
• NCBI Official Symbol: macB
• NCBI Official Synonym Symbols: ECK0870; JW0863; ybjZ
• NCBI Protein Information: fused macrolide transporter subunits of ABC superfamily: ATP-binding component/membrane component
• UniProt Protein Name: Macrolide export ATP-binding/permease protein MacB
• Protein Family: Macrolide export ATP-binding/permease protein
• UniProt Gene Name: macB
• UniProt Synonym Gene Names: ybjZ
• UniProt Entry Name: MACB_ECOLI

NCBI Description

MacABTolC exports 14-15 membered lactone macrolides, e.g. erythromycin. [More information is available at EcoGene: EG13695]. MacB, also known as YbjZ is a half-type ABC protein component of the macrolide efflux transporter complex, MacAB. [More information is available at EcoCyc: G6462].

Uniprot Description

Function: Part of the ABC transporter complex MacAB involved in macrolide export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation. Seems to confer resistance against macrolides composed of 14- and 15-membered lactones but no or weak resistance against 16-membered ones

Probable. Ref.1

Subunit structure: Homodimer

Probable.

Subcellular location: Cell inner membrane; Multi-pass membrane protein Ref.1.

Domain: In MacB the ATP-binding domain (NBD) and the transmembrane domain (TMD) are fused.

Miscellaneous: The complex MacAB-TolC (OMF) forms a single transport channel across the two membranes, allowing direct export of macrolide. This channel is involved in type 1 secretion system.

Sequence similarities: Belongs to the ABC transporter superfamily. Macrolide exporter (TC 3.A.1.122) family. [View classification]Contains 1 ABC transporter domain.