Macrolide-specific efflux protein macA (macA) Recombinant Protein | macA recombinant protein

Recombinant Escherichia coli Macrolide-specific efflux protein macA (macA)

Cat. Num. AAA1216638

• Gene Names: macA; ECK0869; JW0862; ybjY
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Macrolide-specific efflux protein macA (macA); Recombinant Escherichia coli Macrolide-specific efflux protein macA (macA); macA recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 32-371, full length protein
• Sequence: PVPTYQTLIVRPGDLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIKEVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTKAVSQQDLDTAATEMAVKQAQIGTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLADMSAMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYYARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETREREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ
• Sequence Length: 340

• Species: Escherichia coli (strain K12)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 90111182
• NCBI GeneID: 947322
• NCBI Accession #: NP_415399.4
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P75830
• Molecular Weight: 40,624 Da
• NCBI Official Full Name: macrolide transporter membrane fusion protein (MFP) component
• NCBI Official Symbol: macA
• NCBI Official Synonym Symbols: ECK0869; JW0862; ybjY
• NCBI Protein Information: macrolide transporter membrane fusion protein (MFP) component
• UniProt Protein Name: Macrolide export protein MacA
• Protein Family: Maleylacetate reductase
• UniProt Gene Name: macA
• UniProt Synonym Gene Names: ybjY

NCBI Description

OmpF consistently co-purifies with MacA; MacA stimulates MacB ATPase activity (Tikhonova, 2007). [More information is available at EcoGene: EG13694]. MacA is a peripheral membrane protein of the MFP family and a component of the MacAB macrolide efflux transporter complex. [More information is available at EcoCyc: G6461].

Uniprot Description

Part of the tripartite efflux system MacAB-TolC. MacA stimulates the ATPase activity of MacB by promoting the closed ATP-bound state of MacB, increases the capacity of MacB to bind macrolides such as erythromycin, and provides a physical link between MacB and TolC. When overexpressed, the system confers resistance against macrolides composed of 14- and 15-membered lactones but no or weak resistance against 16-membered ones. In addition, MacA binds tightly rough-core lipopolysaccharide (R-LPS), suggesting that the system could also transport R-LPS or a similar glycolipid.