Human lumican ELISA Kit | LUM elisa kit

Human lumican, LUM ELISA Kit

Cat. Num. AAA701117

• Gene Names: LUM; LDC; SLRR2D
• Reactivity: Human
• Synonyms: lumican; Human lumican; LUM ELISA Kit; LDC; SLRR2D; lumican proteoglycan; LUM elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human LUM. No significant cross-reactivity or interference between human LUM and analogues was observed.
• Sequence Length: 338

• Samples: Serum, urine, cell culture supernates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.625 ng/ml-40 ng/ml
• Sensitivity: < 0.156 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LUM elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LUM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LUM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LUM is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LUM bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4505047
• NCBI GeneID: 4060
• NCBI Accession #: NP_002336.1
• NCBI GenBank Nucleotide #: NM_002345.3
• UniProt Accession #: P51884; Q96QM7; B2R6R5
• Molecular Weight: 38,429 Da
• NCBI Official Full Name: lumican
• NCBI Official Synonym Full Names: lumican
• NCBI Official Symbol: LUM
• NCBI Official Synonym Symbols: LDC; SLRR2D
• NCBI Protein Information: lumican; KSPG lumican; lumican proteoglycan; keratan sulfate proteoglycan lumican
• UniProt Protein Name: Lumican
• Protein Family: Lumican
• UniProt Gene Name: LUM
• UniProt Synonym Gene Names: LDC; SLRR2D; KSPG lumican
• UniProt Entry Name: LUM_HUMAN

NCBI Description

This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family that includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. In these bifunctional molecules, the protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. Lumican is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. [provided by RefSeq, Jul 2008]

Uniprot Description

LUM: a member of the small leucine-rich proteoglycan (SLRP) family that includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. In these bifunctional molecules, the protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. Lumican is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. [provided by RefSeq, Jul 2008]

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q21.33

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; lysosomal lumen; fibrillar collagen; Golgi lumen; extracellular region

Molecular Function: collagen binding; protein binding; extracellular matrix structural constituent

Biological Process: keratan sulfate metabolic process; extracellular matrix organization and biogenesis; collagen fibril organization; glycosaminoglycan metabolic process; visual perception; cartilage development; carbohydrate metabolic process; keratan sulfate biosynthetic process; pathogenesis; positive regulation of transcription from RNA polymerase II promoter; keratan sulfate catabolic process; response to organic cyclic substance