Sheep Lymphotactin ELISA Kit | LTN elisa kit

Sheep Lymphotactin ELISA Kit

Cat. Num. AAA738520

• Gene Names: XCL1; LTN; ATAC; LPTN; SCM1; SCM-1; SCM1A; SCYC1; SCM-1a
• Reactivity: Sheep
• Synonyms: Lymphotactin; Sheep Lymphotactin ELISA Kit; LTN elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This LTN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep LTN. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LTN elisa kit

Principle of the Assay: LTN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LTN antibody and an LTN-HRP conjugate. The assay sample and buffer are incubated together with LTN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LTN concentration since LTN from samples and LTN-HRP conjugate compete for the anti-LTN antibody binding site. Since the number of sites is limited, as more sites are occupied by LTN from the sample, fewer sites are left to bind LTN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LTN concentration in each sample is interpolated from this standard curve.

Product Categories/Family for LTN elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 4506853
• NCBI GeneID: 6375
• NCBI Accession #: NP_002986.1
• NCBI GenBank Nucleotide #: NM_002995.2
• Molecular Weight: 12,517 Da
• NCBI Official Full Name: lymphotactin
• NCBI Official Synonym Full Names: chemokine (C motif) ligand 1
• NCBI Official Symbol: XCL1
• NCBI Official Synonym Symbols: LTN; ATAC; LPTN; SCM1; SCM-1; SCM1A; SCYC1; SCM-1a
• NCBI Protein Information: lymphotactin; SCM-1-alpha; lymphotaxin; cytokine SCM-1; c motif chemokine 1; XC chemokine ligand 1; single cysteine motif 1a; small-inducible cytokine C1; small inducible cytokine subfamily C, member 1 (lymphotactin)
• UniProt Protein Name: Lymphotactin
• Protein Family: Lymphotactin
• UniProt Gene Name: XCL1
• UniProt Synonym Gene Names: LTN; SCYC1
• UniProt Entry Name: XCL1_HUMAN

NCBI Description

This antimicrobial gene encodes a member of the chemokine superfamily. Chemokines function in inflammatory and immunological responses, inducing leukocyte migration and activation. The encoded protein is a member of the C-chemokine subfamily, retaining only two of four cysteines conserved in other chemokines, and is thought to be specifically chemotactic for T cells. This gene and a closely related family member are located on the long arm of chromosome 1. [provided by RefSeq, Sep 2014]

Uniprot Description

XCL1: Chemotactic activity for lymphocytes but not for monocytes or neutrophils. Belongs to the intercrine gamma family.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: extracellular space; extracellular region

Molecular Function: protein homodimerization activity; chemokine activity; chemokine receptor binding

Biological Process: neutrophil chemotaxis; negative regulation of T-helper 1 type immune response; positive regulation of T cell mediated cytotoxicity; negative regulation of transcription factor activity; response to virus; positive regulation of T cell cytokine production; positive regulation of leukocyte chemotaxis; negative regulation of T cell cytokine production; signal transduction; positive regulation of interleukin-10 production; negative regulation of interleukin-2 production; cell-cell signaling; release of sequestered calcium ion into cytosol; negative regulation of interferon-gamma production; regulation of inflammatory response; immune response; positive regulation of release of sequestered calcium ion into cytosol; negative regulation of transcription, DNA-dependent