Canine Leukotriene C4 ELISA Kit | LTC4 elisa kit

Canine Leukotriene C4 ELISA Kit

Cat. Num. AAA739485

• Reactivity: Canine
• Synonyms: Leukotriene C4; Canine Leukotriene C4 ELISA Kit; LTC4 elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 50-1000pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This LTC4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine LTC4. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LTC4 elisa kit

Principle of the Assay: LTC4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LTC4 antibody and an LTC4-HRP conjugate. The assay sample and buffer are incubated together with LTC4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LTC4 concentration since LTC4 from samples and LTC4-HRP conjugate compete for the anti-LTC4 antibody binding site. Since the number of sites is limited, as more sites are occupied by LTC4 from the sample, fewer sites are left to bind LTC4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LTC4 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for LTC4 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 22035629
• NCBI GeneID: 4056
• NCBI Accession #: NP_665874.1
• NCBI GenBank Nucleotide #: NM_145867.1
• Molecular Weight: 16,567 Da
• NCBI Official Full Name: leukotriene C4 synthase
• NCBI Official Synonym Full Names: leukotriene C4 synthase
• NCBI Official Symbol: LTC4S
• NCBI Protein Information: leukotriene C4 synthase; LTC4 synthase
• UniProt Protein Name: Leukotriene C4 synthase
• Protein Family: Leukotriene C4 synthase
• UniProt Gene Name: LTC4S
• UniProt Synonym Gene Names: LTC4 synthase
• UniProt Entry Name: LTC4S_HUMAN

NCBI Description

The MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) family includes a number of human proteins, several of which are involved the production of leukotrienes. This gene encodes an enzyme that catalyzes the first step in the biosynthesis of cysteinyl leukotrienes, potent biological compounds derived from arachidonic acid. Leukotrienes have been implicated as mediators of anaphylaxis and inflammatory conditions such as human bronchial asthma. This protein localizes to the nuclear envelope and adjacent endoplasmic reticulum. [provided by RefSeq, Jul 2008]

Uniprot Description

LTC4S: Catalyzes the conjugation of leukotriene A4 with reduced glutathione to form leukotriene C4. Defects in LTC4S are the cause of leukotriene C4 synthase deficiency (LTC4 synthase deficiency). LTC4 synthase deficiency is a fatal neurometabolic developmental disorder. It is associated with muscular hypotonia, psychomotor retardation, failure to thrive, and microcephaly. Belongs to the MAPEG family.

Protein type: Membrane protein, integral; EC 4.4.1.20; Endoplasmic reticulum; Membrane protein, multi-pass; Lipid Metabolism - arachidonic acid; Lyase; Nuclear envelope

Chromosomal Location of Human Ortholog: 5q35

Cellular Component: nuclear outer membrane; endoplasmic reticulum membrane; intracellular membrane-bound organelle; endoplasmic reticulum; integral to membrane; nuclear envelope

Molecular Function: protein binding; glutathione transferase activity; leukotriene-C4 synthase activity; enzyme activator activity; glutathione peroxidase activity; lipid binding

Biological Process: leukotriene biosynthetic process; positive regulation of catalytic activity; lipoxygenase pathway; arachidonic acid metabolic process; leukotriene metabolic process

Disease: Leukotriene C4 Synthase Deficiency