Autoinducer 2 kinase LsrK (lsrK) Recombinant Protein | lsrK recombinant protein
Recombinant Escherichia coli Autoinducer 2 kinase LsrK (lsrK)
NCBI and Uniprot Product Information
NCBI Description
LsrK phosphorylates AI-2; phospo-AI-2 is retained in the cytoplasm. lsr operon induction by exogenous AI-2 requires LsrK, indicating phospho-AI-2, or a derivative, is the LsrR inducer. [More information is available at EcoGene: EG13804]. Based on its similarity to LsrK from Salmonella typhimurium , LsrK is predicted to phosphorylate the quorum-sensing autoinducer molecule AI-2 (shown for S. t. in ). [More information is available at EcoCyc: G6798].
Uniprot Description
Catalyzes the phosphorylation of autoinducer-2 (AI-2) to phospho-AI-2, which subsequently inactivates the transcriptional regulator LsrR and leads to the transcription of the lsr operon. Phosphorylates the ring-open form of (S)-4,5-dihydroxypentane-2,3-dione (DPD), which is the precursor to all AI-2 signaling molecules, at the C5 position. Required for the regulation of the lsr operon and many other genes.
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Product Notes
The lsrK lsrk (Catalog #AAA1216043) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-530, full length protein. The amino acid sequence is listed below: MARLFTLSES KYYLMALDAG TGSIRAVIFD LEGNQIAVGQ AEWRHLAVPD VPGSMEFDLN KNWQLACECM RQALHNAGIA PEYIAAVSAC SMREGIVLYN NEGAPIWACA NVDARAAREV SELKELHNNT FENEVYRATG QTLALSAIPR LLWLAHHRSD IYRQASTITM ISDWLAYMLS GELAVDPSNA GTTGLLDLTT RDWKPALLDM AGLRADILSP VKETGTLLGV VSSQAAELCG LKAGTPVVVG GGDVQLGCLG LGVVRPAQTA VLGGTFWQQV VNLAAPVTDP EMNVRVNPHV IPGMVQAESI SFFTGLTMRW FRDAFCAEEK LIAERLGIDT YTLLEEMASR VPPGSWGVMP IFSDRMRFKT WYHAAPSFIN LSIDPDKCNK ATLFRALEEN AAIVSACNLQ QIADFSNIHP SSLVFAGGGS KGKLWSQILA DVSGLPVNIP VVKEATALGC AIAAGVGAGI FSSMAETGER LVRWERTHTP DPEKHELYQD SRDKWQAVYQ DQLGLVDHGL TTSLWKAPGL. It is sometimes possible for the material contained within the vial of "Autoinducer 2 kinase LsrK (lsrK), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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