Leucine-rich repeat and WD repeat-containing protein 1 (LRWD1) Recombinant Protein | LRWD1 recombinant protein

Recombinant Human Leucine-rich repeat and WD repeat-containing protein 1 (LRWD1)

Cat. Num. AAA1471819

• Gene Names: LRWD1; ORCA; CENP-33
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Leucine-rich repeat and WD repeat-containing protein 1 (LRWD1); Recombinant Human Leucine-rich repeat and WD repeat-containing protein 1 (LRWD1); LRWD1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-647, Full length protein
• Sequence: MGPLSARLLMQRGRPKSDRLGKIRSLDLSGLELLSEHLDPKLLCRLTQLQELDLSNNHLETLPDNLGLSHLRVLRCANNQLGDVTALCQFPKLEELSLEGNPFLTVNDNLKVSFLLPTLRKVNGKDASSTYSQVENLNRELTSRVTAHWEKFMATLGPEEEAEKAQADFVKSAVRDVRYGPESLSEFTQWRVRMISEELVAASRTQVQKANSPEKPPEAGAAHKPRARLAALKRPDDVPLSLSPSKRACASPSAQVEGSPVAGSDGSQPAVKLEPLHFLQCHSKNNSPQDLETQLWACAFEPAWEEGATSQTVATCGGEAVCVIDCQTGIVLHKYKAPGEEFFSVAWTALMVVTQAGHKKRWSVLAAAGLRGLVRLLHVRAGFCCGVIRAHKKAIATLCFSPAHETHLFTASYDKRIILWDIGVPNQDYEFQASQLLTLDTTSIPLRLCPVASCPDARLLAGCEGGCCCWDVRLDQPQKRRVCEVEFVFSEGSEASGRRVDGLAFVNEDIVASKGSGLGTICLWSWRQTWGGRGSQSTVAVVVLARLQWSSTELAYFSLSACPDKGIVLCGDEEGNVWLYDVSNILKQPPLLPAALQAPTQILKWPQPWALGQVVTKTMVNTVVANASFTYLTALTDSNIVAIWGRM
• Sequence Length: 647

• Species: Human
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 959241447
• NCBI GeneID: 222229
• NCBI Accession #: NP_001304650.1
• NCBI GenBank Nucleotide #: NM_001317721.1
• UniProt Accession #: Q9UFC0; Q8N0T9; Q8WV43; Q96GJ2; A8K4K2; B2R9G2
• Molecular Weight: 70,861 Da
• NCBI Official Full Name: leucine-rich repeat and WD repeat-containing protein 1 isoform 2
• NCBI Official Synonym Full Names: leucine rich repeats and WD repeat domain containing 1
• NCBI Official Symbol: LRWD1
• NCBI Official Synonym Symbols: ORCA; CENP-33
• NCBI Protein Information: leucine-rich repeat and WD repeat-containing protein 1
• UniProt Protein Name: Leucine-rich repeat and WD repeat-containing protein 1
• Protein Family: Leucine-rich repeat and WD repeat-containing protein
• UniProt Gene Name: LRWD1
• UniProt Synonym Gene Names: CENP-33; ORC-associated protein; ORCA

NCBI Description

The protein encoded by this gene interacts with components of the origin recognition complex (ORC) and regulates the formation of the prereplicative complex. The encoded protein stabilizes the ORC and therefore aids in DNA replication. This protein is required for the G1/S phase transition of the cell cycle. In addition, the encoded protein binds to trimethylated histone H3 in heterochromatin and recruits the ORC and lysine methyltransferases, which help maintain the repressive heterochromatic state. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2015]

Uniprot Description

Required for G1/S transition. Recruits and stabilizes the origin recognition complex (ORC) onto chromatin during G1 to establish pre-replication complex (preRC) and to heterochromatic sites in post-replicated cells. Binds a combination of DNA and histone methylation repressive marks on heterochromatin. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 in a cooperative manner with DNA methylation. Required for silencing of major satellite repeats. May be important ORC2, ORC3 and ORC4 stability.