Human Low Density Lipoprotein Receptor Related Protein 8 ELISA Kit | LRP8 elisa kit

Human Low Density Lipoprotein Receptor Related Protein 8 ELISA Kit

Cat. Num. AAA91423

• Gene Names: LRP8; MCI1; LRP-8; APOER2; HSZ75190
• Reactivity: Human
• Synonyms: Low Density Lipoprotein Receptor Related Protein 8; Human Low Density Lipoprotein Receptor Related Protein 8 ELISA Kit; LRP8 elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human LRP8 and analogues was observed.
• Sequence Length: 963

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 31.2 ng/ml - 1000 ng/ml.
• Sensitivity: 5.0 ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated LRP8 concentrations in Human serum, plasma and other body fluids. Using Purified Human LRP8 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add LRP8 and LRP8 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of LRP8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) are less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for LRP8 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated LRP8 concentrations in Human serum, plasma and other body fluids. Using Purified Human LRP8 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add LRP8 and LRP8 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of LRP8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 61744471
• NCBI GeneID: 7804
• NCBI Accession #: NP_004622.2
• NCBI GenBank Nucleotide #: NM_004631.4
• UniProt Accession #: Q14114; O14968; Q86V27; Q99876; Q9BR78; B1AMT6; B1AMT7; B1AMT8
• Molecular Weight: 105,634 Da
• NCBI Official Full Name: low-density lipoprotein receptor-related protein 8 isoform 1
• NCBI Official Synonym Full Names: low density lipoprotein receptor-related protein 8, apolipoprotein e receptor
• NCBI Official Symbol: LRP8
• NCBI Official Synonym Symbols: MCI1; LRP-8; APOER2; HSZ75190
• NCBI Protein Information: low-density lipoprotein receptor-related protein 8; ApoE receptor 2
• UniProt Protein Name: Low-density lipoprotein receptor-related protein 8
• Protein Family: Low-density lipoprotein receptor-related protein
• UniProt Gene Name: LRP8
• UniProt Synonym Gene Names: APOER2; LRP-8
• UniProt Entry Name: LRP8_HUMAN

NCBI Description

This gene encodes a member of the low density lipoprotein receptor (LDLR) family. Low density lipoprotein receptors are cell surface proteins that play roles in both signal transduction and receptor-mediated endocytosis of specific ligands for lysosomal degradation. The encoded protein plays a critical role in the migration of neurons during development by mediating Reelin signaling, and also functions as a receptor for the cholesterol transport protein apolipoprotein E. Expression of this gene may be a marker for major depressive disorder. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jun 2011]

Uniprot Description

LRP8: Cell surface receptor for Reelin (RELN) and apolipoprotein E (apoE)-containing ligands. LRP8 participates in transmitting the extracellular Reelin signal to intracellular signaling processes, by binding to DAB1 on its cytoplasmic tail. Reelin acts via both the VLDL receptor (VLDLR) and LRP8 to regulate DAB1 tyrosine phosphorylation and microtubule function in neurons. LRP8 has higher affinity for Reelin than VLDLR. LRP8 is thus a key component of the Reelin pathway which governs neuronal layering of the forebrain during embryonic brain development. Binds the endoplasmic reticulum resident receptor-associated protein (RAP). Binds dimers of beta 2-glycoprotein I and may be involved in the suppression of platelet aggregation in the vasculature. Highly expressed in the initial segment of the epididymis, where it affects the functional expression of clusterin and phospholipid hydroperoxide glutathione peroxidase (PHGPx), two proteins required for sperm maturation. May also function as an endocytic receptor. Defects in LRP8 are a cause of myocardial infarction type 1 (MCI1). A condition defined by the irreversible necrosis of heart muscle secondary to prolonged ischemia. Belongs to the LDLR family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: microtubule associated complex; membrane; cell soma; axon; dendrite; postsynaptic density; integral to membrane; extracellular region; plasma membrane; caveola; receptor complex

Molecular Function: very-low-density lipoprotein receptor activity; protein binding; transmembrane receptor activity; kinesin binding; apolipoprotein binding; calcium ion binding; high-density lipoprotein binding

Biological Process: regulation of synaptic transmission; response to drug; receptor-mediated endocytosis; phototransduction, visible light; cytokine and chemokine mediated signaling pathway; activation of CREB transcription factor; endocytosis; negative regulation of transcription from RNA polymerase II promoter; proteolysis; signal transduction; ammon gyrus development; positive regulation of peptidyl-tyrosine phosphorylation; lipid metabolic process; blood coagulation; retinoid metabolic process; layer formation in the cerebral cortex

Disease: Myocardial Infarction, Susceptibility To