Rabbit Leptin receptor ELISA Kit | LR/Ob-R elisa kit

Rabbit Leptin receptor ELISA Kit

Cat. Num. AAA729530

• Gene Names: LEPR; OBR; OB-R; CD295; LEP-R; LEPRD
• Reactivity: Rabbit
• Synonyms: Leptin receptor; Rabbit Leptin receptor ELISA Kit; LR/Ob-R elisa kit

• Reactivity: Rabbit

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 25-500pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This LR/OB-R ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit LR/OB-R. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LR/Ob-R elisa kit

Principle of the assay: LR/OB-R ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LR/OB-R antibody and an LR/OB-R-HRP conjugate. The assay sample and buffer are incubated together with LR/OB-R-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LR/OB-R concentration since LR/OB-R from samples and LR/OB-R-HRP conjugate compete for the anti-LR/OB-R antibody binding site. Since the number of sites is limited, as more sites are occupied by LR/OB-R from the sample, fewer sites are left to bind LR/OB-R-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LR/OB-R concentration in each sample is interpolated from this standard curve.

Product Categories/Family for LR/Ob-R elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 3236286
• NCBI GeneID: 3953
• Molecular Weight: 96,042 Da
• NCBI Official Full Name: leptin receptor
• NCBI Official Synonym Full Names: leptin receptor
• NCBI Official Symbol: LEPR
• NCBI Official Synonym Symbols: OBR; OB-R; CD295; LEP-R; LEPRD
• NCBI Protein Information: leptin receptor; huB219; OB receptor
• UniProt Protein Name: Leptin receptor
• Protein Family: Leptin receptor
• UniProt Gene Name: LEPR
• UniProt Synonym Gene Names: DB; OBR; LEP-R; OB-R
• UniProt Entry Name: LEPR_HUMAN

NCBI Description

The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).[provided by RefSeq, Nov 2010]

Uniprot Description

LEPR: the receptor for leptin (obesity factor). A single-transmembrane-domain receptor of the cytokine receptor family. On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Five alternatively spliced transcript variants have been reported.

Protein type: Receptor, cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p31

Cellular Component: extracellular region; plasma membrane; integral to membrane; receptor complex

Molecular Function: identical protein binding; hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; transmembrane receptor activity

Biological Process: cholesterol metabolic process; cell surface receptor linked signal transduction; negative regulation of hydrolase activity; cytokine and chemokine mediated signaling pathway; multicellular organismal development; negative regulation of gluconeogenesis; energy reserve metabolic process

Disease: Leptin Receptor Deficiency