Human Lipoprotein Lipase ELISA Kit | LPL elisa kit

Human Lipoprotein Lipase ELISA Kit

Cat. Num. AAA19117

• Gene Names: LPL; LIPD; HDLCQ11
• Reactivity: Human
• Synonyms: Lipoprotein Lipase; Human Lipoprotein Lipase ELISA Kit; LPL elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human LPL and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 50U/L-1600U/L.
• Sensitivity: 10U/L.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated LPL concentrations in Human serum, plasma and other body fluids. Using Purified Human LPL antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add LPL and LPL antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of LPL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for LPL elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated LPL concentrations in Human serum, plasma and other body fluids. Using Purified Human LPL antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add LPL and LPL antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of LPL in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4557727
• NCBI GeneID: 4023
• NCBI Accession #: NP_000228.1
• NCBI GenBank Nucleotide #: NM_000237.2
• UniProt Accession #: P06858; Q16282; Q16283; Q96FC4; B2R5T9
• Molecular Weight: 53,162 Da
• NCBI Official Full Name: lipoprotein lipase
• NCBI Official Synonym Full Names: lipoprotein lipase
• NCBI Official Symbol: LPL
• NCBI Official Synonym Symbols: LIPD; HDLCQ11
• NCBI Protein Information: lipoprotein lipase
• UniProt Protein Name: Lipoprotein lipase
• Protein Family: Lipoprotein
• UniProt Gene Name: LPL
• UniProt Synonym Gene Names: LIPD; LPL
• UniProt Entry Name: LIPL_HUMAN

NCBI Description

LPL encodes lipoprotein lipase, which is expressed in heart, muscle, and adipose tissue. LPL functions as a homodimer, and has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Severe mutations that cause LPL deficiency result in type I hyperlipoproteinemia, while less extreme mutations in LPL are linked to many disorders of lipoprotein metabolism. [provided by RefSeq, Jul 2008]

Uniprot Description

LPL: The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium. Defects in LPL are the cause of lipoprotein lipase deficiency (LPL deficiency); also known as familial chylomicronemia or hyperlipoproteinemia type I. LPL deficiency chylomicronemia is a recessive disorder usually manifesting in childhood. On a normal diet, patients often present with abdominal pain, hepatosplenomegaly, lipemia retinalis, eruptive xanthomata, and massive hypertriglyceridemia, sometimes complicated with acute pancreatitis. Belongs to the AB hydrolase superfamily. Lipase family.

Protein type: Membrane protein, GPI anchor; EC 3.1.1.34; Phospholipase; Lipid Metabolism - glycerolipid

Chromosomal Location of Human Ortholog: 8p22

Cellular Component: extracellular matrix; extracellular space; chylomicron; cell surface; plasma membrane; extracellular region

Molecular Function: heparin binding; triacylglycerol lipase activity; protein binding; lipoprotein lipase activity; apolipoprotein binding; phospholipase activity; receptor binding; triglyceride binding

Biological Process: response to drug; phototransduction, visible light; triacylglycerol metabolic process; phospholipid metabolic process; triacylglycerol catabolic process; lipoprotein metabolic process; triacylglycerol biosynthetic process; response to cold; retinoid metabolic process; fatty acid biosynthetic process

Disease: Hyperlipoproteinemia, Type I; Hyperlipidemia, Familial Combined