LON recombinant protein
Recombinant E Coli LON Protein
Gene Names
lon; capR; deg; dir; ECK0433; JW0429; lonA; lopA; muc
Reactivity
E Coli
Applications
ELISA, Western Blot, SDS-Page
Purity
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Synonyms
LON; Recombinant E Coli LON Protein; capR; deg; lopA; muc; Lon protease; ATP-dependent protease La; LON recombinant protein
Host
E Coli
Reactivity
E Coli
Purity/Purification
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Form/Format
Supplied as a 0.2 muM filtered solution of 50mM Tris-HCl, 100mM KCl, 10% Glycerol, pH 7.5.
Sequence
MGNPERSERI EIPVLPLRDV VVYPHMVIPL FVGREKSIRC LEAAMDHDKK IMLVAQKEAS TDEPGVNDLF TVGTVASILQ MLKLPDGTVK VLVEGLQRAR ISALSDNGEH FSAKAEYLES PTIDEREQEV LVRTAISQFE GYIKLNKKIP PEVLTSLNSI DDPARLADTI AAHMPLKLAD KQSVLEMSDV NERLEYLMAM MESEIDLLQV EKRIRNRVKK QMEKSQREYY LNEQMKAIQK ELGEMDDAPD ENEALKRKID AAKMPKEAKE KAEAELQKLK MMSPMSAEAT VVRGYIDWMV QVPWNARSKV KKDLRQAQEI LDTDHYGLER VKDRILEYLA VQSRVNKIKG PILCLVGPPG VGKTSLGQSI AKATGRKYVR MALGGVRDEA EIRGHRRTYI GSMPGKLIQK MAKVGVKNPL FLLDEIDKMS SDMRGDPASA LLEVLDPEQN VAFSDHYLEV DYDLSDVMFV ATSNSMNIPA PLLDRMEVIR LSGYTEDEKL NIAKRHLLPK QIERNALKKG ELTVDDSAII GIIRYYTREA GVRGLEREIS KLCRKAVKQL LLDKSLKHIE INGDNLHDYL GVQRFDYGRA DNENRVGQVT GLAWTEVGGD LLTIETACVP GKGKLTYTGS LGEVMQESIQ AALTVVRARA EKLGINPDFY EKRDIHVHVP EGATPKDGPS AGIAMCTALV SCLTGNPVRA DVAMTGEITL RGQVLPIGGL KEKLLAAHRG GIKTVLIPFE NKRDLEEIPD NVIADLDIHP VKRIEEVLTL ALQNEPSGMQ VVTAKLEHHH HHH
Sequence Length
784
Applicable Applications for LON recombinant protein
ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
Endotoxin
Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
Directions for Use
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Preparation and Storage
Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.
Related Product Information for LON recombinant protein
Recombinant E Coli LON Protein is produced by our E.coli expression system and the target gene encoding Asn2-Lys784 is expressed with a 6His tag at the C-terminus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
87,438 Da
NCBI Official Full Name
DNA-binding ATP-dependent protease La
NCBI Official Symbol
lon
NCBI Official Synonym Symbols
capR; deg; dir; ECK0433; JW0429; lonA; lopA; muc
NCBI Protein Information
DNA-binding ATP-dependent protease La
UniProt Protein Name
Lon protease
Protein Family
UniProt Gene Name
lon
UniProt Entry Name
LON_ECOLI
NCBI Description
Lon dependent lethality is due to induction of yefM-yoeb toxin/antitoxin pair. [More information is available at EcoGene: EG10542]. Lon is an ATP-dependent protease responsible for degradation of misfolded proteins as well as a number of rapidly degraded regulatory proteins. [More information is available at EcoCyc: EG10542].
Uniprot Description
ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins, including some antitoxins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner. Endogenous substrates include the regulatory proteins RcsA and SulA, the transcriptional activator SoxS, UmuD and antitoxins CcdA, HipB and MazE (PubMed:8022284, PubMed:16460757, PubMed:22720069, PubMed:24375411). Its overproduction specifically inhibits translation through at least two different pathways, one of them being the YoeB-YefM toxin-antitoxin system (PubMed:15009896).
Research Articles on LON
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Product Notes
The LON lon (Catalog #AAA8249399) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The Recombinant E Coli LON Protein reacts with E Coli and may cross-react with other species as described in the data sheet. AAA Biotech's LON can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS). Researchers should empirically determine the suitability of the LON lon for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGNPERSERI EIPVLPLRDV VVYPHMVIPL FVGREKSIRC LEAAMDHDKK IMLVAQKEAS TDEPGVNDLF TVGTVASILQ MLKLPDGTVK VLVEGLQRAR ISALSDNGEH FSAKAEYLES PTIDEREQEV LVRTAISQFE GYIKLNKKIP PEVLTSLNSI DDPARLADTI AAHMPLKLAD KQSVLEMSDV NERLEYLMAM MESEIDLLQV EKRIRNRVKK QMEKSQREYY LNEQMKAIQK ELGEMDDAPD ENEALKRKID AAKMPKEAKE KAEAELQKLK MMSPMSAEAT VVRGYIDWMV QVPWNARSKV KKDLRQAQEI LDTDHYGLER VKDRILEYLA VQSRVNKIKG PILCLVGPPG VGKTSLGQSI AKATGRKYVR MALGGVRDEA EIRGHRRTYI GSMPGKLIQK MAKVGVKNPL FLLDEIDKMS SDMRGDPASA LLEVLDPEQN VAFSDHYLEV DYDLSDVMFV ATSNSMNIPA PLLDRMEVIR LSGYTEDEKL NIAKRHLLPK QIERNALKKG ELTVDDSAII GIIRYYTREA GVRGLEREIS KLCRKAVKQL LLDKSLKHIE INGDNLHDYL GVQRFDYGRA DNENRVGQVT GLAWTEVGGD LLTIETACVP GKGKLTYTGS LGEVMQESIQ AALTVVRARA EKLGINPDFY EKRDIHVHVP EGATPKDGPS AGIAMCTALV SCLTGNPVRA DVAMTGEITL RGQVLPIGGL KEKLLAAHRG GIKTVLIPFE NKRDLEEIPD NVIADLDIHP VKRIEEVLTL ALQNEPSGMQ VVTAKLEHHH HHH. It is sometimes possible for the material contained within the vial of "LON, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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